Experiment set10IT077 for Escherichia coli BW25113

LB Anaerobic with Spiramycin 0.000125 mM

Group: stress
Media: LB + Spiramycin (0.125 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 12 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms

SEED Subsystems

Subsystem	#Specific
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	2
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Fermentations: Mixed acid	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Respiratory dehydrogenases 1	1
Ribosome biogenesis bacterial	1
Trehalose Uptake and Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tyrosine metabolism
• Glycolysis / Gluconeogenesis
• Fatty acid metabolism
• Ubiquinone and menaquinone biosynthesis
• Pyrimidine metabolism
• Glycine, serine and threonine metabolism
• Histidine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Glycerophospholipid metabolism
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• 3-Chloroacrylic acid degradation
• Retinol metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Metabolism of xenobiotics by cytochrome P450
• Insect hormone biosynthesis
• Drug metabolism - cytochrome P450
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
acetaldehyde biosynthesis I	1	1	1
glycerol-3-phosphate shuttle	2	2	1
glycerol-3-phosphate to hydrogen peroxide electron transport	2	2	1
glycerophosphodiester degradation	2	2	1
nitrate reduction IX (dissimilatory)	2	2	1
ethanol degradation I	2	2	1
glycerol-3-phosphate to fumarate electron transfer	2	2	1
pseudouridine degradation	2	2	1
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
pyruvate fermentation to ethanol II	2	1	1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer	2	1	1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer	2	1	1
sn-glycerol 3-phosphate anaerobic respiration	3	3	1
pyruvate fermentation to ethanol I	3	3	1
pyruvate fermentation to ethanol III	3	3	1
ethanol degradation II	3	3	1
glycerol degradation I	3	3	1
L-isoleucine degradation II	3	2	1
L-leucine degradation III	3	2	1
L-valine degradation II	3	2	1
acrylate degradation II	3	2	1
L-methionine degradation III	3	1	1
glycerol and glycerophosphodiester degradation	4	4	1
phytol degradation	4	3	1
salidroside biosynthesis	4	2	1
L-tyrosine degradation III	4	2	1
L-phenylalanine degradation III	4	2	1
cytidine-5'-diphosphate-glycerol biosynthesis	4	1	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
ethanolamine utilization	5	5	1
acetylene degradation (anaerobic)	5	4	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
(S)-propane-1,2-diol degradation	5	3	1
phenylethanol biosynthesis	5	2	1
noradrenaline and adrenaline degradation	13	4	2
3-methylbutanol biosynthesis (engineered)	7	6	1
serotonin degradation	7	3	1
butanol and isobutanol biosynthesis (engineered)	8	2	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	8	1
superpathway of Clostridium acetobutylicum solventogenic fermentation	13	7	1
3-hydroxypropanoate cycle	13	7	1
glyoxylate assimilation	13	5	1
L-tryptophan degradation V (side chain pathway)	13	1	1
mixed acid fermentation	16	16	1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	17	9	1
heterolactic fermentation	18	16	1
3-hydroxypropanoate/4-hydroxybutanate cycle	18	10	1
superpathway of the 3-hydroxypropanoate cycle	18	7	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	19	1
superpathway of anaerobic sucrose degradation	19	17	1
superpathway of N-acetylneuraminate degradation	22	22	1