Experiment set10IT064 for Escherichia coli BW25113

Compare to:

LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

200 most detrimental genes:

  gene name fitness t score description  
b2346 vacJ +4.7 34.2 predicted lipoprotein (NCBI) compare
b3193 yrbD +4.6 32.1 predicted ABC-type organic solvent transporter (NCBI) compare
b3192 yrbC +4.6 38.1 predicted ABC-type organic solvent transporter (NCBI) compare
b3194 yrbE +4.6 35.6 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3195 yrbF +4.5 36.3 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3191 yrbB +4.2 26.1 orf, hypothetical protein (VIMSS) compare
b0632 dacA +3.8 24.0 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3026 qseC +3.8 14.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1049 mdoH +3.4 24.3 glucosyltransferase MdoH (NCBI) compare
b1048 mdoG +3.3 21.8 glucan biosynthesis protein, periplasmic (NCBI) compare
b3786 rffE +2.9 23.6 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b0688 pgm +2.9 11.2 phosphoglucomutase (NCBI) compare
b1097 yceG +2.9 16.5 predicted aminodeoxychorismate lyase (NCBI) compare
b1095 fabF +2.9 19.9 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b3787 rffD +2.8 21.9 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3627 rfaI +2.8 19.7 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b3794 rffM +2.7 18.0 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1830 prc +2.6 15.4 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3784 rfe +2.6 21.0 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3790 rffC +2.6 13.7 TDP-fucosamine acetyltransferase (RefSeq) compare
b3791 rffA +2.6 15.7 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b4042 dgkA +2.5 6.6 diacylglycerol kinase (NCBI) compare
b3628 rfaB +2.5 18.7 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1187 fadR +2.5 12.4 fatty acid metabolism regulator (NCBI) compare
b3205 yhbJ +2.5 9.6 hypothetical protein (NCBI) compare
b2096 gatY +2.4 8.9 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4481 rffT +2.3 12.7 4-alpha-L-fucosyltransferase (NCBI) compare
b0912 ihfB +2.2 7.7 integration host factor subunit beta (NCBI) compare
b3977 tyrU +2.2 2.7 tRNA-Tyr (NCBI) compare
b3169 nusA +2.2 7.2 transcription elongation factor NusA (NCBI) compare
b1220 ychO +2.1 10.8 predicted invasin (RefSeq) compare
b0759 galE +2.1 10.1 UDP-galactose-4-epimerase (VIMSS) compare
b3688 yidQ +2.1 11.8 orf, hypothetical protein (VIMSS) compare
b1236 galU +2.1 10.0 glucose-1-phosphate uridylyltransferase (NCBI) compare
b4359 mdoB +1.9 15.5 phosphoglycerol transferase I (VIMSS) compare
b3785 wzzE +1.9 8.8 putative transport protein (VIMSS) compare
b1217 chaB +1.7 2.6 cation transport regulator (NCBI) compare
b0143 pcnB +1.7 7.8 poly(A) polymerase I (VIMSS) compare
b2134 pbpG +1.7 11.4 penicillin-binding protein 7 (VIMSS) compare
b0051 ksgA +1.7 7.7 dimethyladenosine transferase (NCBI) compare
b4171 miaA +1.6 2.9 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0418 pgpA +1.5 6.8 phosphatidylglycerophosphatase A (NCBI) compare
b3204 ptsN +1.5 4.3 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2350 yfdG +1.5 6.8 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b0125 hpt +1.5 7.6 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b4438 cyaR +1.5 3.6 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b3465 yhhF +1.5 7.7 predicted methyltransferase (NCBI) compare
b1096 pabC +1.5 4.3 4-amino-4-deoxychorismate lyase (NCBI) compare
b1090 plsX +1.4 2.9 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b0407 yajC +1.4 3.9 preprotein translocase subunit YajC (NCBI) compare
b1279 yciS +1.4 1.9 conserved inner membrane protein (NCBI) compare
b4041 plsB +1.4 3.6 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1304 pspA +1.4 3.8 regulatory protein for phage-shock-protein operon (NCBI) compare
b3164 pnp +1.4 3.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1434 ydcN +1.4 3.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3207 yrbL +1.3 6.0 hypothetical protein (NCBI) compare
b1805 fadD +1.3 8.4 acyl-CoA synthase (NCBI) compare
b2092 gatC +1.3 5.3 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b2093 gatB +1.3 3.9 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b0843 ybjH +1.3 4.1 hypothetical protein (NCBI) compare
b3742 mioC +1.3 5.2 flavodoxin (NCBI) compare
b3203 yhbH +1.3 1.5 predicted ribosome-associated, sigma 54 modulation protein (NCBI) compare
b0122 yacC +1.3 5.5 orf, hypothetical protein (VIMSS) compare
b2263 yfbB +1.3 4.1 predicted peptidase (NCBI) compare
b4558 yifL +1.2 3.7 predicted lipoprotein (NCBI) compare
b3632 rfaQ +1.2 10.3 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0819 ybiS +1.2 7.0 hypothetical protein (NCBI) compare
b1424 mdoD +1.2 5.8 glucan biosynthesis protein, periplasmic (RefSeq) compare
b0247 ykfG +1.2 2.0 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0685 ybfE +1.2 2.4 orf, hypothetical protein (VIMSS) compare
b0678 nagB +1.2 4.6 glucosamine-6-phosphate deaminase (NCBI) compare
b2095 gatZ +1.2 4.9 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b3625 rfaY +1.2 5.3 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1349 recT +1.2 4.8 Rac prophage; recombination and repair protein (NCBI) compare
b3630 rfaP +1.2 8.9 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2684 mprA +1.2 3.3 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1047 mdoC +1.2 7.4 glucans biosynthesis protein (NCBI) compare
b4416 rybA +1.2 2.0 unknown RNA (NCBI) compare
b3289 rsmB +1.2 8.9 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare
b1412 azoR +1.2 2.7 acyl carrier protein phosphodiesterase (NCBI) compare
b4174 hflK +1.1 8.0 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b0920 ycbC +1.1 3.3 conserved inner membrane protein (NCBI) compare
b0209 yafD +1.1 7.5 hypothetical protein (NCBI) compare
b2279 nuoK +1.1 2.1 NADH dehydrogenase subunit K (NCBI) compare
b0889 lrp +1.1 3.2 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2836 aas +1.1 7.6 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI) compare
b4175 hflC +1.1 7.1 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3728 pstS +1.1 3.7 phosphate transporter subunit (NCBI) compare
b2132 bglX +1.1 7.7 beta-D-glucoside glucohydrolase, periplasmic (NCBI) compare
b4392 slt +1.1 8.7 soluble lytic murein transglycosylase (VIMSS) compare
b2094 gatA +1.1 3.5 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b3390 aroK +1.0 2.0 shikimate kinase I (NCBI) compare
b2187 yejL +1.0 2.7 hypothetical protein (NCBI) compare
b1712 ihfA +1.0 1.4 integration host factor subunit alpha (NCBI) compare
b1263 trpD +1.0 3.0 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b2298 yfcC +1.0 6.5 putative S-transferase (VIMSS) compare
b2264 menD +1.0 5.9 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1833 yebS +1.0 4.7 conserved inner membrane protein (NCBI) compare
b2448 yffQ +1.0 2.2 CPZ-55 prophage; predicted protein (RefSeq) compare
b2287 nuoB +1.0 3.3 NADH dehydrogenase subunit B (NCBI) compare
b2814 metZ +1.0 1.6 tRNA-Met (NCBI) compare
b2835 ygeD +1.0 5.3 predicted inner membrane protein (NCBI) compare
b1253 yciA +0.9 1.8 predicted hydrolase (NCBI) compare
b4647 mokA +0.9 0.5 no description compare
b2061 wzb +0.9 2.0 protein-tyrosine phosphatase (NCBI) compare
b3842 rfaH +0.9 4.7 transcriptional activator RfaH (NCBI) compare
b3258 panF +0.9 5.7 sodium/pantothenate symporter (VIMSS) compare
b2570 rseC +0.9 2.6 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b3157 yhbT +0.9 2.4 predicted lipid carrier protein (NCBI) compare
b1296 ycjJ +0.9 3.6 putative amino acid/amine transport protein (VIMSS) compare
b3257 yhdT +0.9 3.5 conserved inner membrane protein (NCBI) compare
b3911 cpxA +0.8 5.7 two-component sensor protein (NCBI) compare
b0376 ampH +0.8 5.7 beta-lactamase/D-alanine carboxypeptidase (NCBI) compare
b3788 rffG +0.8 4.0 dTDP-glucose 4,6-dehydratase (NCBI) compare
b3423 glpR +0.8 4.7 DNA-binding transcriptional repressor (NCBI) compare
b1427 rimL +0.8 2.0 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b0772 ybhC +0.8 6.0 predicted pectinesterase (NCBI) compare
b2951 yggS +0.8 2.6 predicted enzyme (NCBI) compare
b2209 eco +0.8 1.8 ecotin precursor (NCBI) compare
b1526 yneJ +0.8 2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1620 malI +0.8 1.6 DNA-binding transcriptional repressor (NCBI) compare
b1531 marA +0.8 1.7 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b0619 citA +0.8 3.3 sensory histidine kinase in two-component regulatory system with citB (NCBI) compare
b1915 yecF +0.8 3.8 hypothetical protein (NCBI) compare
b1358 ydaT +0.8 1.0 Rac prophage; predicted protein (NCBI) compare
b0080 fruR +0.8 3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3182 dacB +0.8 6.0 D-alanyl-D-alanine carboxypeptidase (NCBI) compare
b2260 menE +0.8 5.0 O-succinylbenzoic acid--CoA ligase (NCBI) compare
b3035 tolC +0.8 4.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b3474 yhhT +0.8 5.1 orf, hypothetical protein (VIMSS) compare
b1949 fliQ +0.8 1.4 flagellar biosynthesis protein Q (NCBI) compare
b1628 rsxB +0.8 2.0 electron transport complex protein RnfB (NCBI) compare
b1907 tyrP +0.8 2.7 tyrosine transporter (NCBI) compare
b1853 yebK +0.8 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3631 rfaG +0.8 6.4 glucosyltransferase I (NCBI) compare
b2531 iscR +0.8 1.2 DNA-binding transcriptional repressor (NCBI) compare
b3009 yghB +0.7 3.9 conserved inner membrane protein (NCBI) compare
b4595 yciY +0.7 1.1 hypothetical protein (RefSeq) compare
b0219 yafV +0.7 2.4 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b1584 speG +0.7 4.3 spermidine N1-acetyltransferase (NCBI) compare
b2377 yfdY +0.7 1.6 predicted inner membrane protein (NCBI) compare
b0908 aroA +0.7 4.2 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b1290 sapF +0.7 1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2419 yfeK +0.7 1.7 hypothetical protein (NCBI) compare
b3806 cyaA +0.7 3.5 adenylate cyclase (NCBI) compare
b3389 aroB +0.7 3.7 3-dehydroquinate synthase (NCBI) compare
b1180 ycgM +0.7 2.5 predicted isomerase/hydrolase (NCBI) compare
b4484 cpxP +0.7 2.3 periplasmic protein combats stress (NCBI) compare
b2682 ygaZ +0.7 3.1 predicted transporter (NCBI) compare
b2083 yegZ +0.7 1.7 orf, hypothetical protein (VIMSS) compare
b1103 ycfF +0.7 0.9 orf, hypothetical protein (VIMSS) compare
b3493 pitA +0.7 4.1 phosphate transporter, low-affinity (NCBI) compare
b0796 ybiH +0.7 2.4 putative transcriptional regulator (VIMSS) compare
b2240 glpT +0.7 5.2 sn-glycerol-3-phosphate transporter (NCBI) compare
b2749 ygbE +0.7 3.7 conserved inner membrane protein (NCBI) compare
b3926 glpK +0.7 5.7 glycerol kinase (NCBI) compare
b4596 yciZ +0.7 1.4 hypothetical protein (RefSeq) compare
b0213 yafS +0.7 3.2 orf, hypothetical protein (VIMSS) compare
b2904 gcvH +0.7 1.0 glycine cleavage system protein H (NCBI) compare
b2927 epd +0.7 4.1 D-erythrose 4-phosphate dehydrogenase (NCBI) compare
b4381 deoC +0.7 3.0 deoxyribose-phosphate aldolase (NCBI) compare
b1901 araF +0.7 2.5 L-arabinose transporter subunit (NCBI) compare
b1953 b1953 +0.7 1.4 orf, hypothetical protein (VIMSS) compare
b3620 rfaF +0.7 3.5 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2282 nuoH +0.7 2.1 NADH dehydrogenase subunit H (NCBI) compare
b3281 aroE +0.7 5.0 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b2911 ssrS +0.7 1.5 6S regulatory RNA (NCBI) compare
b0866 ybjQ +0.7 1.0 hypothetical protein (NCBI) compare
b1731 cedA +0.7 2.2 cell division modulator (RefSeq) compare
b4039 ubiC +0.7 4.3 chorismate lyase (VIMSS) compare
b2189 proL +0.7 1.3 tRNA-Pro (NCBI) compare
b2329 aroC +0.7 2.8 chorismate synthase (NCBI) compare
b1917 yecC +0.7 2.1 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2743 pcm +0.6 2.3 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b2618 b2618 +0.6 2.4 orf, hypothetical protein (VIMSS) compare
b1179 ycgL +0.6 1.4 conserved protein (NCBI) compare
b0191 yaeJ +0.6 2.5 hypothetical protein (NCBI) compare
b2587 kgtP +0.6 2.9 alpha-ketoglutarate transporter (NCBI) compare
b1678 ynhG +0.6 3.9 hypothetical protein (NCBI) compare
b2039 rfbA +0.6 5.2 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b3746 yieN +0.6 4.9 putative 2-component regulator (VIMSS) compare
b4527 b1354 +0.6 1.5 orf, hypothetical protein (VIMSS) compare
b4577 sgrS +0.6 1.6 small antisense RNA (NCBI) compare
b3965 trmA +0.6 4.3 tRNA (uracil-5-)-methyltransferase (NCBI) compare
b1171 b1171 +0.6 1.5 orf, hypothetical protein (VIMSS) compare
b1834 yebT +0.6 3.7 orf, hypothetical protein (VIMSS) compare
b1411 ynbD +0.6 2.6 predicted phosphatase, inner membrane protein (NCBI) compare
b2174 b2174 +0.6 3.3 orf, hypothetical protein (VIMSS) compare
b0431 cyoB +0.6 4.4 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b4573 insZ +0.6 2.6 no description compare
b1360 ydaV +0.6 2.0 Rac prophage; predicted DNA replication protein (NCBI) compare
b1669 ydhT +0.6 1.8 hypothetical protein (NCBI) compare
b2701 mltB +0.6 3.4 membrane-bound lytic murein transglycosylase B (NCBI) compare
b4219 msrA +0.6 2.6 methionine sulfoxide reductase A (NCBI) compare
b1993 cobU +0.6 2.1 adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI) compare
b1582 ynfA +0.6 1.7 hypothetical protein (NCBI) compare
b1693 aroD +0.6 2.3 3-dehydroquinate dehydratase (NCBI) compare
b1684 sufA +0.6 1.6 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b1660 ydhC +0.6 1.7 predicted transporter (NCBI) compare
b1856 yebA +0.6 3.5 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

For 46 genes in this experiment

For stress Vancomycin Hydrochloride from Streptomyces orientalis in Escherichia coli BW25113

For stress Vancomycin Hydrochloride from Streptomyces orientalis across organisms