Experiment set10IT064 for Escherichia coli BW25113

LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

Group: stress
Media: LB + Vancomycin Hydrochloride from Streptomyces orientalis (3.13E-02 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 46 genes in this experiment

For stress Vancomycin Hydrochloride from Streptomyces orientalis in Escherichia coli BW25113

For stress Vancomycin Hydrochloride from Streptomyces orientalis across organisms

SEED Subsystems

Subsystem	#Specific
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	4
Respiratory dehydrogenases 1	3
Glycerol and Glycerol-3-phosphate Uptake and Utilization	2
Iron acquisition in Vibrio	2
KDO2-Lipid A biosynthesis	2
Transport of Iron	2
Beta-Glucoside Metabolism	1
Biotin biosynthesis	1
Campylobacter Iron Metabolism	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Flagellum	1
Glycogen metabolism	1
Maltose and Maltodextrin Utilization	1
Peptidoglycan Biosynthesis	1
Synechocystis experimental	1
Triacylglycerol metabolism	1
Type IV pilus	1
n-Phenylalkanoic acid degradation	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycerophospholipid metabolism
• Nucleotide sugars metabolism
• Fatty acid metabolism
• Ubiquinone and menaquinone biosynthesis
• Starch and sucrose metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Ascorbate and aldarate metabolism
• Puromycin biosynthesis
• Pyrimidine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Aminophosphonate metabolism
• Cyanoamino acid metabolism
• High-mannose type N-glycan biosynthesis
• Glycosaminoglycan degradation
• Glycerolipid metabolism
• alpha-Linolenic acid metabolism
• Sphingolipid metabolism
• Naphthalene and anthracene degradation
• Trinitrotoluene degradation
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
long-chain fatty acid activation	1	1	1
cardiolipin biosynthesis I	3	3	2
cardiolipin biosynthesis II	3	3	2
neolinustatin bioactivation	3	2	2
glycerol-3-phosphate to fumarate electron transfer	2	2	1
glycerophosphodiester degradation	2	2	1
nitrate reduction IX (dissimilatory)	2	2	1
glycerol-3-phosphate to hydrogen peroxide electron transport	2	2	1
glycerol-3-phosphate shuttle	2	2	1
pseudouridine degradation	2	2	1
linustatin bioactivation	4	2	2
γ-linolenate biosynthesis II (animals)	2	1	1
cinnamoyl-CoA biosynthesis	2	1	1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer	2	1	1
lotaustralin degradation	2	1	1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer	2	1	1
linoleate biosynthesis II (animals)	2	1	1
linamarin degradation	2	1	1
glycerol degradation I	3	3	1
sn-glycerol 3-phosphate anaerobic respiration	3	3	1
cardiolipin biosynthesis III	3	3	1
cellulose degradation II (fungi)	3	2	1
3-methyl-branched fatty acid α-oxidation	6	3	2
alkane biosynthesis II	3	1	1
oleate biosynthesis I (plants)	3	1	1
heptadecane biosynthesis	3	1	1
alkane biosynthesis I	3	1	1
glycerol and glycerophosphodiester degradation	4	4	1
starch degradation V	4	3	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
phytol degradation	4	3	1
phosphatidylcholine acyl editing	4	2	1
phospholipid remodeling (phosphatidylethanolamine, yeast)	4	2	1
pinosylvin metabolism	4	1	1
long chain fatty acid ester synthesis (engineered)	4	1	1
wax esters biosynthesis II	4	1	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	3
sporopollenin precursors biosynthesis	18	5	4
CMP-3-deoxy-D-manno-octulosonate biosynthesis	5	5	1
5,6-dehydrokavain biosynthesis (engineered)	10	8	2
peptidoglycan recycling II	10	7	2
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
benzoate biosynthesis III (CoA-dependent, non-β-oxidative)	5	2	1
octane oxidation	5	2	1
coumarin biosynthesis (via 2-coumarate)	5	2	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	2
phosphatidylglycerol biosynthesis II	6	6	1
phosphatidylglycerol biosynthesis I	6	6	1
fatty acid salvage	6	5	1
stearate biosynthesis II (bacteria and plants)	6	5	1
stearate biosynthesis IV	6	4	1
6-gingerol analog biosynthesis (engineered)	6	2	1
α-tomatine degradation	6	1	1
stearate biosynthesis I (animals)	6	1	1
peptidoglycan recycling I	14	14	2
CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis	7	4	1
ceramide degradation by α-oxidation	7	2	1
arachidonate biosynthesis III (6-desaturase, mammals)	7	1	1
capsaicin biosynthesis	7	1	1
icosapentaenoate biosynthesis III (8-desaturase, mammals)	7	1	1
icosapentaenoate biosynthesis II (6-desaturase, mammals)	7	1	1
glycogen degradation I	8	8	1
sucrose biosynthesis II	8	6	1
2-deoxy-D-ribose degradation II	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
benzoate biosynthesis I (CoA-dependent, β-oxidative)	9	4	1
starch degradation II	9	1	1
3-phenylpropanoate degradation	10	4	1
suberin monomers biosynthesis	20	3	2
superpathway of fatty acid biosynthesis II (plant)	43	38	4
mycobactin biosynthesis	11	3	1
firefly bioluminescence	14	2	1
palmitate biosynthesis II (type II fatty acid synthase)	31	29	2
cutin biosynthesis	16	2	1
superpathway of (Kdo)2-lipid A biosynthesis	17	17	1
type I lipoteichoic acid biosynthesis (S. aureus)	17	5	1
superpathway of Kdo2-lipid A biosynthesis	25	24	1
superpathway of fatty acids biosynthesis (E. coli)	53	51	2
superpathway of phospholipid biosynthesis II (plants)	28	10	1
palmitate biosynthesis III	29	21	1
oleate β-oxidation	35	32	1