Experiment set10IT064 for Escherichia coli BW25113

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LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

Group: stress
Media: LB + Vancomycin Hydrochloride from Streptomyces orientalis (3.13E-02 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 48 genes in this experiment

For stress Vancomycin Hydrochloride from Streptomyces orientalis in Escherichia coli BW25113

For stress Vancomycin Hydrochloride from Streptomyces orientalis across organisms

SEED Subsystems

Subsystem #Specific
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 4
Respiratory dehydrogenases 1 3
Glycerol and Glycerol-3-phosphate Uptake and Utilization 2
Iron acquisition in Vibrio 2
KDO2-Lipid A biosynthesis 2
Transport of Iron 2
Beta-Glucoside Metabolism 1
Biotin biosynthesis 1
Campylobacter Iron Metabolism 1
Conserved gene cluster associated with Met-tRNA formyltransferase 1
D-Tagatose and Galactitol Utilization 1
Flagellum 1
Glycogen metabolism 1
Maltose and Maltodextrin Utilization 1
Peptidoglycan Biosynthesis 1
Synechocystis experimental 1
Triacylglycerol metabolism 1
Type IV pilus 1
n-Phenylalkanoic acid degradation 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
long-chain fatty acid activation 1 1 1
cardiolipin biosynthesis I 3 3 2
cardiolipin biosynthesis II 3 3 2
neolinustatin bioactivation 3 2 2
glycerophosphodiester degradation 2 2 1
glycerol-3-phosphate to fumarate electron transfer 2 2 1
pseudouridine degradation 2 2 1
nitrate reduction IX (dissimilatory) 2 2 1
glycerol-3-phosphate to hydrogen peroxide electron transport 2 2 1
glycerol-3-phosphate shuttle 2 2 1
linustatin bioactivation 4 2 2
linamarin degradation 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 1 1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer 2 1 1
cinnamoyl-CoA biosynthesis 2 1 1
lotaustralin degradation 2 1 1
linoleate biosynthesis II (animals) 2 1 1
sn-glycerol 3-phosphate anaerobic respiration 3 3 1
cardiolipin biosynthesis III 3 3 1
glycerol degradation I 3 3 1
cellulose degradation II (fungi) 3 2 1
3-methyl-branched fatty acid α-oxidation 6 3 2
oleate biosynthesis I (plants) 3 1 1
alkane biosynthesis II 3 1 1
alkane biosynthesis I 3 1 1
heptadecane biosynthesis 3 1 1
glycerol and glycerophosphodiester degradation 4 4 1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas) 4 3 1
starch degradation V 4 3 1
phytol degradation 4 3 1
phosphatidylcholine acyl editing 4 2 1
phospholipid remodeling (phosphatidylethanolamine, yeast) 4 2 1
wax esters biosynthesis II 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
pinosylvin metabolism 4 1 1
superpathway of cardiolipin biosynthesis (bacteria) 13 11 3
sporopollenin precursors biosynthesis 18 5 4
CMP-3-deoxy-D-manno-octulosonate biosynthesis 5 5 1
galactitol degradation 5 5 1
5,6-dehydrokavain biosynthesis (engineered) 10 8 2
peptidoglycan recycling II 10 7 2
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
coumarin biosynthesis (via 2-coumarate) 5 2 1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 2 1
octane oxidation 5 2 1
superpathway of phospholipid biosynthesis III (E. coli) 12 12 2
phosphatidylglycerol biosynthesis II 6 6 1
phosphatidylglycerol biosynthesis I 6 6 1
stearate biosynthesis II (bacteria and plants) 6 5 1
fatty acid salvage 6 5 1
stearate biosynthesis IV 6 4 1
6-gingerol analog biosynthesis (engineered) 6 2 1
α-tomatine degradation 6 1 1
stearate biosynthesis I (animals) 6 1 1
peptidoglycan recycling I 14 14 2
CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis 7 4 1
ceramide degradation by α-oxidation 7 2 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
capsaicin biosynthesis 7 1 1
glycogen degradation I 8 8 1
sucrose biosynthesis II 8 6 1
2-deoxy-D-ribose degradation II 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 4 1
starch degradation II 9 1 1
3-phenylpropanoate degradation 10 4 1
suberin monomers biosynthesis 20 3 2
superpathway of fatty acid biosynthesis II (plant) 43 38 4
mycobactin biosynthesis 11 3 1
firefly bioluminescence 14 2 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
cutin biosynthesis 16 2 1
superpathway of (Kdo)2-lipid A biosynthesis 17 17 1
type I lipoteichoic acid biosynthesis (S. aureus) 17 5 1
superpathway of hexitol degradation (bacteria) 18 18 1
superpathway of Kdo2-lipid A biosynthesis 25 24 1
superpathway of fatty acids biosynthesis (E. coli) 53 51 2
superpathway of phospholipid biosynthesis II (plants) 28 10 1
palmitate biosynthesis III 29 21 1
oleate β-oxidation 35 32 1