Experiment set10IT064 for Agrobacterium fabrum C58

methyl-beta-L-arabinopyranoside carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + methyl-beta-L-arabinopyranoside (5 mM), pH=7
Culturing: Agro_ML11, 24-well plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 1/6/22
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 9 genes in this experiment

For carbon source methyl-beta-L-arabinopyranoside in Agrobacterium fabrum C58

For carbon source methyl-beta-L-arabinopyranoside across organisms

SEED Subsystems

Subsystem	#Specific
L-Arabinose utilization	2
Lactose and Galactose Uptake and Utilization	2
Fructooligosaccharides(FOS) and Raffinose Utilization	1
Galactosylceramide and Sulfatide metabolism	1
Maltose and Maltodextrin Utilization	1
Melibiose Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Glycerolipid metabolism
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - globo series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
melibiose degradation	1	1	1
D-galactose degradation II	2	2	1
trehalose degradation VI (periplasmic)	2	1	1
stachyose degradation	7	4	2
D-galactose degradation I (Leloir pathway)	5	3	1