Experiment set10IT060 for Agrobacterium fabrum C58

Methyl-B-D-galactopyranoside carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Methyl-B-D-galactopyranoside (10 mM), pH=7
Culturing: Agro_ML11, 24-well plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 1/6/22
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 12 genes in this experiment

For carbon source Methyl-B-D-galactopyranoside in Agrobacterium fabrum C58

For carbon source Methyl-B-D-galactopyranoside across organisms

SEED Subsystems

Subsystem	#Specific
Glycolate, glyoxylate interconversions	1
Maltose and Maltodextrin Utilization	1
Melibiose Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.