Experiment set10IT059 for Agrobacterium fabrum C58

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DL-glyceraldehyde carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + DL-glyceraldehyde (10 mM), pH=7
Culturing: Agro_ML11, 24-well plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 1/6/22
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 7 genes in this experiment

For carbon source DL-glyceraldehyde in Agrobacterium fabrum C58

For carbon source DL-glyceraldehyde across organisms

SEED Subsystems

Subsystem #Specific
Phosphate metabolism 2
Conenzyme B12 related Hypothetical: Clusters with cobST 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
MLST 1
Pentose phosphate pathway 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
sulfoacetaldehyde degradation III 1 1 1
pentose phosphate pathway (oxidative branch) I 3 3 1
glycerol degradation I 3 2 1
glycerol and glycerophosphodiester degradation 4 3 1
NAD(P)/NADPH interconversion 6 3 1
pentose phosphate pathway 8 8 1
superpathway of glucose and xylose degradation 17 16 1