Experiment set10IT057 for Agrobacterium fabrum C58

Uridine 5-monophosphate disodium salt carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Uridine 5-monophosphate disodium salt (5 mM), pH=7
Culturing: Agro_ML11, 24-well plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 1/6/22
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 10 genes in this experiment

For carbon source Uridine 5-monophosphate disodium salt in Agrobacterium fabrum C58

For carbon source Uridine 5-monophosphate disodium salt across organisms

SEED Subsystems

Subsystem	#Specific
D-ribose utilization	4
Deoxyribose and Deoxynucleoside Catabolism	4
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	2
Chitin and N-acetylglucosamine utilization	1
Entner-Doudoroff Pathway	1
Fermentations: Mixed acid	1
Glycolate, glyoxylate interconversions	1
Methylglyoxal Metabolism	1
Purine conversions	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
Queuosine-Archaeosine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Fatty acid metabolism
• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Tryptophan metabolism
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Limonene and pinene degradation
• Ascorbate and aldarate metabolism
• Purine metabolism
• Glycine, serine and threonine metabolism
• Valine, leucine and isoleucine degradation
• Geraniol degradation
• Lysine biosynthesis
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• beta-Alanine metabolism
• Glycerolipid metabolism
• Pyruvate metabolism
• Naphthalene and anthracene degradation
• 1,2-Dichloroethane degradation
• Propanoate metabolism
• Butanoate metabolism
• Nicotinate and nicotinamide metabolism
• Retinol metabolism
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
acetaldehyde biosynthesis I	1	1	1
ethanol degradation II	3	3	2
adenine and adenosine salvage II	2	2	1
guanine and guanosine salvage II	2	2	1
pyrimidine ribonucleosides salvage II	2	2	1
pyrimidine ribonucleosides salvage III	2	2	1
putrescine degradation V	2	2	1
phytol degradation	4	3	2
putrescine degradation I	2	1	1
pyruvate fermentation to ethanol II	2	1	1
ethanol degradation I	2	1	1
ethylene glycol degradation	2	1	1
ethanol degradation IV	3	3	1
superpathway of guanosine nucleotides degradation (plants)	6	5	2
L-isoleucine degradation II	3	2	1
L-valine degradation II	3	2	1
ethanol degradation III	3	2	1
hypotaurine degradation	3	2	1
putrescine degradation IV	3	2	1
L-leucine degradation III	3	2	1
histamine degradation	3	1	1
pyruvate fermentation to ethanol I	3	1	1
pyruvate fermentation to ethanol III	3	1	1
L-methionine degradation III	3	1	1
noradrenaline and adrenaline degradation	13	4	4
serotonin degradation	7	3	2
guanosine nucleotides degradation II	4	4	1
adenosine nucleotides degradation I	8	7	2
purine nucleotides degradation I (plants)	12	10	3
guanosine nucleotides degradation I	4	3	1
L-tyrosine degradation III	4	2	1
L-phenylalanine degradation III	4	2	1
L-tryptophan degradation X (mammalian, via tryptamine)	4	2	1
fatty acid α-oxidation I (plants)	4	2	1
cytidine-5'-diphosphate-glycerol biosynthesis	4	1	1
D-arabinose degradation II	4	1	1
salidroside biosynthesis	4	1	1
putrescine degradation III	4	1	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
mitochondrial NADPH production (yeast)	5	3	1
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
octane oxidation	5	2	1
dopamine degradation	5	1	1
acetylene degradation (anaerobic)	5	1	1
ethanolamine utilization	5	1	1
phenylethanol biosynthesis	5	1	1
(S)-propane-1,2-diol degradation	5	1	1
superpathway of purines degradation in plants	18	12	3
3-methyl-branched fatty acid α-oxidation	6	3	1
alkane oxidation	6	1	1
3-methylbutanol biosynthesis (engineered)	7	6	1
superpathway of glycol metabolism and degradation	7	4	1
ceramide degradation by α-oxidation	7	2	1
limonene degradation IV (anaerobic)	7	1	1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast)	8	6	1
superpathway of ornithine degradation	8	4	1
butanol and isobutanol biosynthesis (engineered)	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
aromatic biogenic amine degradation (bacteria)	8	1	1
Entner-Doudoroff pathway II (non-phosphorylative)	9	5	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	3	1
superpathway of pyrimidine ribonucleosides salvage	10	8	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	6	1
superpathway of L-arginine and L-ornithine degradation	13	8	1
superpathway of Clostridium acetobutylicum solventogenic fermentation	13	5	1
L-tryptophan degradation V (side chain pathway)	13	1	1
mixed acid fermentation	16	9	1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	17	5	1
heterolactic fermentation	18	12	1
superpathway of anaerobic sucrose degradation	19	13	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	10	1
superpathway of N-acetylneuraminate degradation	22	12	1
superpathway of pentose and pentitol degradation	42	16	1