Experiment set10IT054 for Pseudomonas putida KT2440

Valeric acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Valeric acid (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 4.1 generations
By: Mitchell Thompson on 10/18/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 12 genes in this experiment

For carbon source Valeric acid in Pseudomonas putida KT2440

For carbon source Valeric acid across organisms

SEED Subsystems

Subsystem	#Specific
Phosphonate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Propanoate metabolism
• Fatty acid metabolism
• Valine, leucine and isoleucine degradation
• Geraniol degradation
• Tyrosine metabolism
• beta-Alanine metabolism
• alpha-Linolenic acid metabolism
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Limonene and pinene degradation
• Caprolactam degradation
• Alkaloid biosynthesis II
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adipate degradation	5	5	1
fatty acid salvage	6	6	1
L-leucine degradation I	6	5	1
oleate β-oxidation	35	30	3
2-methyl-branched fatty acid β-oxidation	14	10	1