Experiment set10IT054 for Escherichia coli BW25113

Compare to:

LB Anaerobic with Spiramycin 0.00003125 mM

200 most detrimental genes:

  gene name fitness t score description  
b3977 tyrU +2.0 2.7 tRNA-Tyr (NCBI) compare
b1607 ydgC +1.5 3.2 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b2259 pmrD +1.2 1.6 polymyxin resistance protein B (VIMSS) compare
b1865 nudB +1.2 2.0 dATP pyrophosphohydrolase (NCBI) compare
b1358 ydaT +1.2 1.4 Rac prophage; predicted protein (NCBI) compare
b2281 nuoI +1.2 3.8 NADH dehydrogenase subunit I (NCBI) compare
b1524 yneH +1.2 1.4 predicted glutaminase (NCBI) compare
b1217 chaB +1.1 1.4 cation transport regulator (NCBI) compare
b4042 dgkA +1.1 3.1 diacylglycerol kinase (NCBI) compare
b2058 wcaB +1.1 1.6 predicted acyl transferase (NCBI) compare
b4041 plsB +1.1 3.2 glycerol-3-phosphate acyltransferase (VIMSS) compare
b2614 grpE +1.1 2.0 heat shock protein (NCBI) compare
b1446 ydcY +1.1 2.8 hypothetical protein (NCBI) compare
b3205 yhbJ +1.0 4.1 hypothetical protein (NCBI) compare
b2282 nuoH +1.0 3.8 NADH dehydrogenase subunit H (NCBI) compare
b4533 ynfO +1.0 2.4 Qin prophage; predicted protein (NCBI) compare
b2688 gshA +1.0 6.3 glutamate--cysteine ligase (NCBI) compare
b1187 fadR +1.0 5.6 fatty acid metabolism regulator (NCBI) compare
b0125 hpt +0.9 4.6 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b0464 acrR +0.9 6.6 DNA-binding transcriptional repressor (NCBI) compare
b1289 ycjD +0.9 2.9 hypothetical protein (NCBI) compare
b0418 pgpA +0.9 5.2 phosphatidylglycerophosphatase A (NCBI) compare
b2906 visC +0.9 4.3 hypothetical protein (NCBI) compare
b3119 tdcR +0.9 2.8 threonine dehydratase operon activator protein (VIMSS) compare
b3806 cyaA +0.9 4.7 adenylate cyclase (NCBI) compare
b1103 ycfF +0.9 1.1 orf, hypothetical protein (VIMSS) compare
b1312 ycjP +0.9 3.5 predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2279 nuoK +0.8 1.5 NADH dehydrogenase subunit K (NCBI) compare
b2904 gcvH +0.8 2.8 glycine cleavage system protein H (NCBI) compare
b1613 manA +0.8 1.8 mannose-6-phosphate isomerase (VIMSS) compare
b3844 fre +0.8 5.1 NAD(P)H-flavin reductase (NCBI) compare
b3257 yhdT +0.8 2.6 conserved inner membrane protein (NCBI) compare
b1684 sufA +0.8 2.0 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b3181 greA +0.8 2.4 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3192 yrbC +0.8 5.4 predicted ABC-type organic solvent transporter (NCBI) compare
b3193 yrbD +0.8 5.2 predicted ABC-type organic solvent transporter (NCBI) compare
b2096 gatY +0.7 3.8 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1095 fabF +0.7 4.3 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b4241 treR +0.7 5.9 trehalose repressor (NCBI) compare
b0439 lon +0.7 3.3 DNA-binding ATP-dependent protease La (NCBI) compare
b3195 yrbF +0.7 5.2 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2175 spr +0.7 3.1 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3169 nusA +0.7 1.8 transcription elongation factor NusA (NCBI) compare
b1628 rsxB +0.7 1.5 electron transport complex protein RnfB (NCBI) compare
b1038 csgF +0.7 2.1 predicted transport protein (NCBI) compare
b0682 ybfN +0.7 2.8 predicted lipoprotein (NCBI) compare
b2276 nuoN +0.7 3.6 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3191 yrbB +0.7 4.9 orf, hypothetical protein (VIMSS) compare
b2083 yegZ +0.7 1.2 orf, hypothetical protein (VIMSS) compare
b3026 qseC +0.7 2.0 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1667 ydhR +0.6 1.4 hypothetical protein (NCBI) compare
b3194 yrbE +0.6 5.0 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2318 truA +0.6 2.1 tRNA pseudouridine synthase A (NCBI) compare
b4418 sraB +0.6 1.7 unknown RNA (NCBI) compare
b0955 ycbZ +0.6 3.9 predicted peptidase (NCBI) compare
b1266 yciV +0.6 2.4 hypothetical protein (NCBI) compare
b2187 yejL +0.6 2.1 hypothetical protein (NCBI) compare
b1243 oppA +0.6 2.8 oligopeptide transporter subunit (NCBI) compare
b1891 flhC +0.6 2.1 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b1090 plsX +0.6 2.9 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b0796 ybiH +0.6 2.1 putative transcriptional regulator (VIMSS) compare
b1143 ymfI +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b0209 yafD +0.6 3.5 hypothetical protein (NCBI) compare
b0211 mltD +0.6 3.0 predicted membrane-bound lytic murein transglycosylase D (NCBI) compare
b2229 b2229 +0.6 2.3 orf, hypothetical protein (VIMSS) compare
b2951 yggS +0.6 2.7 predicted enzyme (NCBI) compare
b2283 nuoG +0.6 3.7 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b1526 yneJ +0.6 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1185 dsbB +0.6 1.9 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1253 yciA +0.6 1.8 predicted hydrolase (NCBI) compare
b2708 gutQ +0.6 2.4 orf, hypothetical protein (VIMSS) compare
b0404 yajB +0.6 2.6 hypothetical protein (NCBI) compare
b2278 nuoL +0.6 4.0 NADH dehydrogenase subunit L (NCBI) compare
b2280 nuoJ +0.6 2.5 NADH dehydrogenase subunit J (NCBI) compare
b1728 ydjM +0.6 1.9 predicted inner membrane protein regulated by LexA (RefSeq) compare
b1582 ynfA +0.6 1.3 hypothetical protein (NCBI) compare
b0504 ybbS +0.6 2.3 DNA-binding transcriptional activator of the allD operon (NCBI) compare
b1846 yebE +0.6 2.2 hypothetical protein (NCBI) compare
b2743 pcm +0.6 2.4 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b1075 flgD +0.6 2.2 flagellar basal body rod modification protein D (NCBI) compare
b2063 yegH +0.6 2.7 putative transport protein (VIMSS) compare
b1805 fadD +0.6 4.0 acyl-CoA synthase (NCBI) compare
b2659 b2659 +0.6 0.9 orf, hypothetical protein (VIMSS) compare
b1271 yciK +0.6 1.9 short chain dehydrogenase (NCBI) compare
b0632 dacA +0.6 3.6 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b2962 yggX +0.6 1.7 hypothetical protein (NCBI) compare
b2419 yfeK +0.6 1.0 hypothetical protein (NCBI) compare
b3293 yhdN +0.6 2.5 hypothetical protein (NCBI) compare
b1915 yecF +0.6 3.1 hypothetical protein (NCBI) compare
b1412 azoR +0.6 1.7 acyl carrier protein phosphodiesterase (NCBI) compare
b1632 rsxE +0.6 3.5 NADH-ubiquinone oxidoreductase (NCBI) compare
b0942 ycbU +0.6 2.2 predicted fimbrial-like adhesin protein (NCBI) compare
b3863 polA +0.6 0.6 DNA polymerase I (NCBI) compare
b3388 damX +0.6 4.6 hypothetical protein (NCBI) compare
b1926 fliT +0.5 2.0 predicted chaperone (NCBI) compare
b0912 ihfB +0.5 2.1 integration host factor subunit beta (NCBI) compare
b0965 b0965 +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b0926 ycbK +0.5 3.2 hypothetical protein (NCBI) compare
b3555 yiaG +0.5 1.9 predicted transcriptional regulator (NCBI) compare
b0431 cyoB +0.5 4.3 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b1171 b1171 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b1630 rsxD +0.5 2.9 electron transport complex protein RnfD (NCBI) compare
b0430 cyoC +0.5 1.8 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b2080 b2080 +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b2346 vacJ +0.5 3.6 predicted lipoprotein (NCBI) compare
b2093 gatB +0.5 1.9 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b2061 wzb +0.5 1.3 protein-tyrosine phosphatase (NCBI) compare
b2286 nuoC +0.5 2.8 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b0802 ybiJ +0.5 1.3 hypothetical protein (NCBI) compare
b2684 mprA +0.5 1.7 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b4692 oweE +0.5 1.3 no description compare
b3791 rffA +0.5 3.3 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0407 yajC +0.5 1.4 preprotein translocase subunit YajC (NCBI) compare
b0018 mokC +0.5 0.9 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b2426 ucpA +0.5 1.2 putative oxidoreductase (VIMSS) compare
b2448 yffQ +0.5 1.7 CPZ-55 prophage; predicted protein (RefSeq) compare
b1640 ydhH +0.5 1.9 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b2948 yqgE +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b1853 yebK +0.5 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2768 ygcP +0.5 1.9 predicted anti-terminator regulatory protein (NCBI) compare
b2835 ygeD +0.5 3.3 predicted inner membrane protein (NCBI) compare
b0858 ybjO +0.5 1.2 predicted inner membrane protein (NCBI) compare
b3068 ygjF +0.5 1.4 G/U mismatch-specific DNA glycosylase (NCBI) compare
b3029 ygiN +0.5 1.1 quinol monooxygenase (NCBI) compare
b2258 b2258 +0.5 0.9 putative transport/receptor protein (VIMSS) compare
b2517 yfgB +0.5 2.9 predicted enzyme (NCBI) conserved
b0746 valZ +0.5 0.6 tRNA-Val (NCBI) compare
b1686 ydiI +0.5 1.1 hypothetical protein (NCBI) compare
b1712 ihfA +0.5 0.7 integration host factor subunit alpha (NCBI) compare
b4386 lplA +0.5 3.5 lipoate-protein ligase A (NCBI) compare
b0817 mntR +0.5 1.3 DNA-binding transcriptional regulator of mntH (NCBI) compare
b4381 deoC +0.5 1.8 deoxyribose-phosphate aldolase (NCBI) compare
b3203 yhbH +0.5 1.1 predicted ribosome-associated, sigma 54 modulation protein (NCBI) compare
b0798 ybiA +0.5 0.8 hypothetical protein (NCBI) compare
b2502 ppx +0.5 2.8 exopolyphosphatase (NCBI) compare
b0613 citG +0.5 1.7 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b1348 lar +0.5 0.9 Rac prophage; restriction alleviation protein (NCBI) compare
b0059 hepA +0.5 4.0 ATP-dependent helicase HepA (NCBI) compare
b0630 lipB +0.5 0.9 protein of lipoate biosynthesis (VIMSS) compare
b2291 yfbR +0.5 1.5 hypothetical protein (NCBI) compare
b4598 yncL +0.5 1.2 hypothetical protein (RefSeq) compare
b2947 gshB +0.5 2.0 glutathione synthetase (NCBI) compare
b1946 fliN +0.5 1.6 flagellar motor switch protein (NCBI) compare
b1811 yoaH +0.5 1.8 hypothetical protein (NCBI) compare
b4695 ykgT +0.5 0.8 no description compare
b3258 panF +0.5 3.1 sodium/pantothenate symporter (VIMSS) compare
b2912 ygfA +0.5 1.6 putative ligase (VIMSS) compare
b2836 aas +0.5 3.5 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI) compare
b1841 yobA +0.5 0.8 hypothetical protein (NCBI) compare
b1584 speG +0.5 2.8 spermidine N1-acetyltransferase (NCBI) compare
b2015 yeeY +0.5 2.5 putative transcriptional regulator LYSR-type (VIMSS) compare
b1424 mdoD +0.5 2.4 glucan biosynthesis protein, periplasmic (RefSeq) compare
b1246 oppD +0.5 2.5 oligopeptide transporter ATP-binding component (NCBI) compare
b3860 dsbA +0.5 2.8 periplasmic protein disulfide isomerase I (NCBI) compare
b2489 hyfI +0.5 2.1 hydrogenase 4, Fe-S subunit (NCBI) compare
b1306 pspC +0.5 1.5 DNA-binding transcriptional activator (NCBI) compare
b3790 rffC +0.5 2.4 TDP-fucosamine acetyltransferase (RefSeq) compare
b0143 pcnB +0.5 2.5 poly(A) polymerase I (VIMSS) compare
b1827 kdgR +0.4 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b4648 ysaC +0.4 1.6 no description compare
b1622 malY +0.4 2.0 bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI) compare
b4594 ymgJ +0.4 1.0 hypothetical protein (RefSeq) compare
b2277 nuoM +0.4 2.5 NADH dehydrogenase subunit M (NCBI) compare
b1349 recT +0.4 2.0 Rac prophage; recombination and repair protein (NCBI) compare
b2690 yqaB +0.4 1.6 predicted hydrolase (NCBI) compare
b2047 wcaJ +0.4 1.5 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b0655 gltI +0.4 1.5 glutamate and aspartate transporter subunit (NCBI) compare
b2443 yffL +0.4 1.6 CPZ-55 prophage; predicted protein (NCBI) compare
b0852 rimK +0.4 2.1 ribosomal protein S6 modification protein (NCBI) compare
b0487 cueR +0.4 1.5 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b2343 b2343 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b0428 cyoE +0.4 2.1 protoheme IX farnesyltransferase (NCBI) compare
b1049 mdoH +0.4 3.0 glucosyltransferase MdoH (NCBI) compare
b4581 ybeM +0.4 2.4 no description compare
b4513 kdpF +0.4 1.4 potassium ion accessory transporter subunit (NCBI) compare
b2782 chpA +0.4 1.4 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b4438 cyaR +0.4 0.7 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b3221 yhcH +0.4 1.8 hypothetical protein (NCBI) compare
b1514 lsrC +0.4 1.0 AI2 transporter (NCBI) compare
b1462 b1462 +0.4 0.7 orf, hypothetical protein (VIMSS) compare
b0991 ymcE +0.4 1.6 cold shock gene (NCBI) compare
b2673 nrdH +0.4 1.2 glutaredoxin-like protein (NCBI) compare
b1418 cybB +0.4 1.4 cytochrome b(561) (VIMSS) compare
b2263 yfbB +0.4 1.0 predicted peptidase (NCBI) compare
b3786 rffE +0.4 3.4 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b4460 araH +0.4 1.2 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b1553 ydfP +0.4 1.2 Qin prophage; conserved protein (NCBI) compare
b1778 yeaA +0.4 1.1 methionine sulfoxide reductase B (NCBI) compare
b2298 yfcC +0.4 2.6 putative S-transferase (VIMSS) compare
b2221 atoD +0.4 1.3 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b1336 ydaH +0.4 1.7 putative pump protein (transport) (VIMSS) compare
b2113 mrp +0.4 2.0 putative ATPase (VIMSS) compare
b3033 yqiB +0.4 1.8 predicted dehydrogenase (NCBI) compare
b2156 lysP +0.4 2.8 lysine transporter (NCBI) compare
b0228 yafM +0.4 2.1 hypothetical protein (NCBI) compare
b2240 glpT +0.4 2.9 sn-glycerol-3-phosphate transporter (NCBI) compare
b2923 argO +0.4 2.1 arginine exporter protein (NCBI) compare
b3206 npr +0.4 1.5 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1221 narL +0.4 1.9 DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI) compare
b3834 yigP +0.4 1.9 hypothetical protein (NCBI) compare


Specific Phenotypes

For 2 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms