Experiment set10IT053 for Escherichia coli BW25113

Compare to:

LB Anaerobic with Spiramycin 0.000125 mM

200 most detrimental genes:

  gene name fitness t score description  
b0464 acrR +4.6 28.4 DNA-binding transcriptional repressor (NCBI) compare
b3169 nusA +3.5 10.8 transcription elongation factor NusA (NCBI) compare
b1187 fadR +3.1 14.2 fatty acid metabolism regulator (NCBI) compare
b0955 ycbZ +3.0 18.0 predicted peptidase (NCBI) compare
b0439 lon +2.9 13.6 DNA-binding ATP-dependent protease La (NCBI) compare
b3390 aroK +2.5 6.3 shikimate kinase I (NCBI) compare
b3493 pitA +2.5 16.7 phosphate transporter, low-affinity (NCBI) compare
b2904 gcvH +2.3 2.0 glycine cleavage system protein H (NCBI) compare
b1049 mdoH +2.3 16.2 glucosyltransferase MdoH (NCBI) compare
b3205 yhbJ +2.2 8.8 hypothetical protein (NCBI) compare
b1048 mdoG +2.1 12.1 glucan biosynthesis protein, periplasmic (NCBI) compare
b1865 nudB +2.1 4.3 dATP pyrophosphohydrolase (NCBI) compare
b3806 cyaA +2.0 10.4 adenylate cyclase (NCBI) compare
b4042 dgkA +1.9 5.6 diacylglycerol kinase (NCBI) compare
b2517 yfgB +1.8 10.5 predicted enzyme (NCBI) conserved
b2688 gshA +1.7 10.2 glutamate--cysteine ligase (NCBI) compare
b2175 spr +1.7 5.8 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b1530 marR +1.7 5.1 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0685 ybfE +1.6 6.7 orf, hypothetical protein (VIMSS) compare
b0926 ycbK +1.6 5.2 hypothetical protein (NCBI) compare
b1185 dsbB +1.5 5.5 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b0912 ihfB +1.5 4.1 integration host factor subunit beta (NCBI) compare
b0125 hpt +1.5 6.6 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b2279 nuoK +1.5 3.0 NADH dehydrogenase subunit K (NCBI) compare
b2951 yggS +1.4 7.4 predicted enzyme (NCBI) compare
b1628 rsxB +1.4 2.8 electron transport complex protein RnfB (NCBI) compare
b4241 treR +1.4 10.8 trehalose repressor (NCBI) compare
b2531 iscR +1.3 2.5 DNA-binding transcriptional repressor (NCBI) compare
b2947 gshB +1.3 6.2 glutathione synthetase (NCBI) compare
b1582 ynfA +1.3 2.5 hypothetical protein (NCBI) compare
b1217 chaB +1.2 1.7 cation transport regulator (NCBI) compare
b4592 yccB +1.2 1.5 hypothetical protein (RefSeq) compare
b3688 yidQ +1.2 5.8 orf, hypothetical protein (VIMSS) compare
b1630 rsxD +1.1 3.1 electron transport complex protein RnfD (NCBI) compare
b2684 mprA +1.1 4.7 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b4246 pyrL +1.1 3.4 pyrBI operon leader peptide (NCBI) compare
b2259 pmrD +1.1 1.4 polymyxin resistance protein B (VIMSS) compare
b2516 yfgA +1.1 5.9 hypothetical protein (NCBI) compare
b3792 wzxE +1.1 7.3 O-antigen translocase (NCBI) compare
b3863 polA +1.0 1.2 DNA polymerase I (NCBI) compare
b1412 azoR +1.0 3.6 acyl carrier protein phosphodiesterase (NCBI) compare
b1095 fabF +1.0 6.0 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b2285 nuoE +1.0 2.0 NADH dehydrogenase subunit E (NCBI) compare
b2618 b2618 +1.0 3.4 orf, hypothetical protein (VIMSS) compare
b1103 ycfF +1.0 1.8 orf, hypothetical protein (VIMSS) compare
b0247 ykfG +0.9 1.7 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b3261 fis +0.9 2.1 DNA-binding protein Fis (NCBI) compare
b0209 yafD +0.9 5.3 hypothetical protein (NCBI) compare
b3388 damX +0.9 7.7 hypothetical protein (NCBI) compare
b3180 yhbY +0.9 4.7 predicted RNA-binding protein (NCBI) compare
b2096 gatY +0.9 4.7 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4558 yifL +0.9 4.4 predicted lipoprotein (NCBI) compare
b2083 yegZ +0.9 2.1 orf, hypothetical protein (VIMSS) compare
b4527 b1354 +0.9 2.7 orf, hypothetical protein (VIMSS) compare
b2282 nuoH +0.9 3.1 NADH dehydrogenase subunit H (NCBI) compare
b0143 pcnB +0.9 4.1 poly(A) polymerase I (VIMSS) compare
b2263 yfbB +0.8 2.1 predicted peptidase (NCBI) compare
b1134 ymfB +0.8 2.5 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b0560 nohB +0.8 2.6 DLP12 prophage; DNA packaging protein (NCBI) compare
b2287 nuoB +0.8 3.2 NADH dehydrogenase subunit B (NCBI) compare
b1842 holE +0.8 1.5 DNA polymerase III, theta subunit (NCBI) compare
b1284 yciT +0.8 2.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b0051 ksgA +0.8 3.5 dimethyladenosine transferase (NCBI) compare
b1434 ydcN +0.8 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b2708 gutQ +0.8 4.2 orf, hypothetical protein (VIMSS) compare
b3181 greA +0.8 2.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2262 menB +0.8 2.4 naphthoate synthase (NCBI) compare
b2281 nuoI +0.8 2.3 NADH dehydrogenase subunit I (NCBI) compare
b1090 plsX +0.8 1.6 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b4171 miaA +0.8 2.5 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b2906 visC +0.8 4.0 hypothetical protein (NCBI) compare
b1446 ydcY +0.8 1.8 hypothetical protein (NCBI) compare
b3465 yhhF +0.8 4.6 predicted methyltransferase (NCBI) compare
b4041 plsB +0.7 2.0 glycerol-3-phosphate acyltransferase (VIMSS) compare
b3204 ptsN +0.7 2.5 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2450 yffS +0.7 1.1 CPZ-55 prophage; predicted protein (RefSeq) compare
b1632 rsxE +0.7 4.1 NADH-ubiquinone oxidoreductase (NCBI) compare
b1070 flgN +0.7 1.3 export chaperone for FlgK and FlgL (NCBI) compare
b2835 ygeD +0.7 4.9 predicted inner membrane protein (NCBI) compare
b2659 b2659 +0.7 2.0 orf, hypothetical protein (VIMSS) compare
b1712 ihfA +0.7 1.0 integration host factor subunit alpha (NCBI) compare
b4596 yciZ +0.7 1.2 hypothetical protein (RefSeq) compare
b1253 yciA +0.7 1.6 predicted hydrolase (NCBI) compare
b2319 usg +0.7 2.8 hypothetical protein (NCBI) compare
b0852 rimK +0.7 3.4 ribosomal protein S6 modification protein (NCBI) compare
b1805 fadD +0.7 4.3 acyl-CoA synthase (NCBI) compare
b3257 yhdT +0.7 2.8 conserved inner membrane protein (NCBI) compare
b4175 hflC +0.7 4.3 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2912 ygfA +0.7 1.9 putative ligase (VIMSS) compare
b2061 wzb +0.7 1.6 protein-tyrosine phosphatase (NCBI) compare
b0542 renD +0.7 1.3 orf, hypothetical protein (VIMSS) compare
b0974 hyaC +0.7 2.2 hydrogenase 1, b-type cytochrome subunit (NCBI) compare
b3250 mreC +0.7 0.5 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b0263 afuB +0.7 2.2 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b4538 yoeF +0.7 1.3 hypothetical protein (RefSeq) compare
b4438 cyaR +0.6 1.3 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b3057 bacA +0.6 3.7 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b0407 yajC +0.6 1.6 preprotein translocase subunit YajC (NCBI) compare
b3926 glpK +0.6 5.4 glycerol kinase (NCBI) compare
b1977 asnT +0.6 1.8 tRNA-Asn (NCBI) compare
b1424 mdoD +0.6 3.0 glucan biosynthesis protein, periplasmic (RefSeq) compare
b1563 relE +0.6 1.3 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b2095 gatZ +0.6 3.3 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b2502 ppx +0.6 4.1 exopolyphosphatase (NCBI) compare
b1573 ydfC +0.6 1.2 Qin prophage; predicted protein (RefSeq) compare
b2280 nuoJ +0.6 2.5 NADH dehydrogenase subunit J (NCBI) compare
b1290 sapF +0.6 1.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2240 glpT +0.6 4.6 sn-glycerol-3-phosphate transporter (NCBI) compare
b2683 ygaH +0.6 3.5 predicted inner membrane protein (NCBI) compare
b4529 ydbJ +0.6 0.8 hypothetical protein (NCBI) compare
b2329 aroC +0.6 2.9 chorismate synthase (NCBI) compare
b0430 cyoC +0.6 2.9 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b2066 udk +0.6 3.0 uridine/cytidine kinase (VIMSS) compare
b3203 yhbH +0.6 1.1 predicted ribosome-associated, sigma 54 modulation protein (NCBI) compare
b0678 nagB +0.6 2.8 glucosamine-6-phosphate deaminase (NCBI) compare
b1915 yecF +0.6 3.0 hypothetical protein (NCBI) compare
b1635 gst +0.6 2.3 glutathionine S-transferase (NCBI) compare
b3398 yrfF +0.6 2.3 predicted inner membrane protein (NCBI) compare
b4359 mdoB +0.6 5.0 phosphoglycerol transferase I (VIMSS) compare
b0982 yccY +0.6 2.0 putative phosphatase (VIMSS) compare
b2318 truA +0.6 1.2 tRNA pseudouridine synthase A (NCBI) compare
b4063 soxR +0.6 1.8 DNA-binding transcriptional dual regulator, Fe-S center for redox-sensing (NCBI) compare
b2836 aas +0.6 4.1 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI) compare
b1811 yoaH +0.6 1.9 hypothetical protein (NCBI) compare
b2283 nuoG +0.6 3.5 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b4435 isrC +0.6 1.5 unknown RNA (NCBI) compare
b1832 yebR +0.6 2.5 free methionine-(R)-sulfoxide reductase (RefSeq) compare
b1742 b1742 +0.6 2.0 orf, hypothetical protein (VIMSS) compare
b2421 cysM +0.6 2.2 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b4370 leuQ +0.6 1.7 tRNA-Leu (NCBI) compare
b1293 sapB +0.6 0.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1220 ychO +0.6 2.9 predicted invasin (RefSeq) compare
b2092 gatC +0.6 3.2 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b1661 cfa +0.6 2.7 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b2156 lysP +0.6 3.8 lysine transporter (NCBI) compare
b3977 tyrU +0.6 0.6 tRNA-Tyr (NCBI) compare
b0920 ycbC +0.5 1.5 conserved inner membrane protein (NCBI) compare
b4513 kdpF +0.5 1.0 potassium ion accessory transporter subunit (NCBI) compare
b1186 nhaB +0.5 3.5 NhaB sodium/proton transporter (Katherine Huang) compare
b1251 yciI +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b3611 yibN +0.5 1.9 predicted rhodanese-related sulfurtransferase (NCBI) compare
b4557 yidD +0.5 1.6 hypothetical protein (NCBI) compare
b2782 chpA +0.5 1.9 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b2286 nuoC +0.5 2.2 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b3258 panF +0.5 3.6 sodium/pantothenate symporter (VIMSS) compare
b1349 recT +0.5 3.1 Rac prophage; recombination and repair protein (NCBI) compare
b4577 sgrS +0.5 1.4 small antisense RNA (NCBI) compare
b1096 pabC +0.5 2.5 4-amino-4-deoxychorismate lyase (NCBI) compare
b3781 trxA +0.5 1.9 thioredoxin 1 (VIMSS) compare
b0885 aat +0.5 2.7 leucyl/phenylalanyl-tRNA--protein transferase (NCBI) compare
b2966 yqgA +0.5 2.3 predicted inner membrane protein (NCBI) compare
b3149 yraO +0.5 2.8 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1868 yecE +0.5 1.8 hypothetical protein (NCBI) compare
b0428 cyoE +0.5 3.1 protoheme IX farnesyltransferase (NCBI) compare
b4695 ykgT +0.5 1.1 no description compare
b1839 yebY +0.5 1.7 hypothetical protein (NCBI) compare
b0998 torD +0.5 0.8 chaperone protein TorD (NCBI) compare
b0418 pgpA +0.5 2.6 phosphatidylglycerophosphatase A (NCBI) compare
b1524 yneH +0.5 0.5 predicted glutaminase (NCBI) compare
b2276 nuoN +0.5 2.6 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b2134 pbpG +0.5 2.9 penicillin-binding protein 7 (VIMSS) compare
b3555 yiaG +0.5 1.8 predicted transcriptional regulator (NCBI) compare
b1729 ydjN +0.5 1.6 predicted transporter (NCBI) compare
b1466 narW +0.5 2.1 nitrate reductase 2 (NRZ), delta subunit (assembly subunit) (NCBI) compare
b3221 yhcH +0.5 1.3 hypothetical protein (NCBI) compare
b0865 ybjP +0.5 1.4 predicted lipoprotein (NCBI) compare
b4508 peaD +0.5 1.4 putative sensory transduction regulator (VIMSS) compare
b2289 lrhA +0.5 3.4 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b2284 nuoF +0.5 2.3 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b0856 potH +0.5 2.3 putrescine transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2261 menC +0.5 2.5 O-succinylbenzoate synthase (NCBI) compare
b0250 ykfB +0.5 2.3 CP4-6 prophage; predicted protein (NCBI) compare
b4174 hflK +0.5 3.5 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1926 fliT +0.5 1.9 predicted chaperone (NCBI) compare
b1188 ycgB +0.5 2.7 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
b3029 ygiN +0.5 1.5 quinol monooxygenase (NCBI) compare
b1780 yeaD +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b1138 ymfE +0.5 1.0 e14 prophage; predicted inner membrane protein (NCBI) compare
b4409 blr +0.5 2.4 beta-lactam resistance membrane protein (NCBI) compare
b2277 nuoM +0.5 2.5 NADH dehydrogenase subunit M (NCBI) compare
b3742 mioC +0.5 2.2 flavodoxin (NCBI) compare
b1180 ycgM +0.5 2.0 predicted isomerase/hydrolase (NCBI) compare
b0977 hyaF +0.5 1.5 protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI) compare
b3836 b3836 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b1856 yebA +0.5 2.3 orf, hypothetical protein (VIMSS) compare
b1244 oppB +0.5 3.2 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b1684 sufA +0.5 1.3 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b2911 ssrS +0.5 1.0 6S regulatory RNA (NCBI) compare
b2278 nuoL +0.5 3.3 NADH dehydrogenase subunit L (NCBI) compare
b1704 aroH +0.5 2.3 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible (NCBI) compare
b2946 yggJ +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b3527 yhjJ +0.5 4.2 predicted zinc-dependent peptidase (NCBI) compare
b0753 ybgS +0.5 2.1 hypothetical protein (NCBI) compare
b0493 ybbO +0.5 1.9 short chain dehydrogenase (NCBI) compare
b1556 b1556 +0.5 1.0 orf, hypothetical protein (VIMSS) compare
b4390 nadR +0.5 3.5 probable nadAB transcriptional regulator (VIMSS) compare
b1697 ydiQ +0.5 2.5 hypothetical protein (NCBI) compare
b2467 yffH +0.5 2.4 predicted NUDIX hydrolase (NCBI) compare
b2264 menD +0.5 2.6 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b0334 prpD +0.5 2.2 2-methylcitrate dehydratase (NCBI) compare


Specific Phenotypes

For 13 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms