Experiment set10IT053 for Escherichia coli BW25113

LB Anaerobic with Spiramycin 0.000125 mM

Group: stress
Media: LB + Spiramycin (0.125 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 13 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms

SEED Subsystems

Subsystem	#Specific
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	3
Respiratory dehydrogenases 1	3
Glycerol and Glycerol-3-phosphate Uptake and Utilization	2
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Ribosome biogenesis bacterial	1
Trehalose Uptake and Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycerophospholipid metabolism
• Ubiquinone and menaquinone biosynthesis
• Pyrimidine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Aminophosphonate metabolism
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Sphingolipid metabolism
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycerol-3-phosphate to hydrogen peroxide electron transport	2	2	1
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
pseudouridine degradation	2	2	1
glycerol-3-phosphate shuttle	2	2	1
glycerophosphodiester degradation	2	2	1
glycerol-3-phosphate to fumarate electron transfer	2	2	1
nitrate reduction IX (dissimilatory)	2	2	1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer	2	1	1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer	2	1	1
sn-glycerol 3-phosphate anaerobic respiration	3	3	1
glycerol degradation I	3	3	1
cardiolipin biosynthesis I	3	3	1
cardiolipin biosynthesis II	3	3	1
glycerol and glycerophosphodiester degradation	4	4	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	2
superpathway of phospholipid biosynthesis III (E. coli)	12	12	1