Experiment set10IT052 for Agrobacterium fabrum C58

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Propan-1-ol carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Propan-1-ol (5 mM), pH=7
Culturing: Agro_ML11, 24-well plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 1/6/22
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 9 genes in this experiment

For carbon source Propan-1-ol in Agrobacterium fabrum C58

For carbon source Propan-1-ol across organisms

SEED Subsystems

Subsystem #Specific
Propionyl-CoA to Succinyl-CoA Module 3
Serine-glyoxylate cycle 2
Cobalamin synthesis 1
Coenzyme B12 biosynthesis 1
Glutathione: Non-redox reactions 1
tRNA splicing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
propanoyl CoA degradation I 3 3 3
2-oxobutanoate degradation I 4 3 3
aminopropanol phosphate biosynthesis I 2 1 1
conversion of succinate to propanoate 3 1 1
anaerobic energy metabolism (invertebrates, mitochondrial) 10 8 3
pyruvate fermentation to propanoate I 7 4 2
4-hydroxy-2-nonenal detoxification 4 1 1
3-hydroxypropanoate cycle 13 8 3
pentachlorophenol degradation 10 4 2
superpathway of L-methionine salvage and degradation 16 8 3
L-glutamate degradation VIII (to propanoate) 11 4 2
superpathway of anaerobic energy metabolism (invertebrates) 17 13 3
3-hydroxypropanoate/4-hydroxybutanate cycle 18 11 3
superpathway of the 3-hydroxypropanoate cycle 18 8 3
methylaspartate cycle 19 12 3
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 5 2
crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) 14 3 2
glutathione-mediated detoxification I 8 3 1
gliotoxin biosynthesis 9 2 1
glutathione-mediated detoxification II 9 1 1
ethylmalonyl-CoA pathway 11 3 1
indole glucosinolate activation (intact plant cell) 12 3 1
camalexin biosynthesis 12 2 1
superpathway of L-threonine metabolism 18 11 1
adenosylcobalamin biosynthesis II (aerobic) 33 29 1
adenosylcobalamin biosynthesis I (anaerobic) 36 26 1
mycolate biosynthesis 205 21 1
superpathway of mycolate biosynthesis 239 22 1