Experiment set10IT048 for Pseudomonas putida KT2440

D-Mannose carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + D-Mannose (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 4.2 generations
By: Mitchell Thompson on 10/18/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 13 genes in this experiment

For carbon source D-Mannose in Pseudomonas putida KT2440

For carbon source D-Mannose across organisms

SEED Subsystems

Subsystem	#Specific
Fructose utilization	2
Fructose and Mannose Inducible PTS	1
Mannitol Utilization	1
Phosphate metabolism	1
Purine conversions	1
Sialic Acid Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Purine metabolism
• Starch and sucrose metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adenosine nucleotides degradation III	1	1	1
N-acetylneuraminate and N-acetylmannosamine degradation II	3	1	1
sulfoquinovose degradation III	4	2	1
sulfoquinovose degradation VI	5	2	1
sulfoquinovose degradation I	5	1	1
sulfoquinovose degradation V	7	1	1
glycolysis IV	10	8	1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	18	1