Experiment set10IT048 for Agrobacterium fabrum C58
Glycerol carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + Glycerol (10 mM), pH=7
Culturing: Agro_ML11, 24-well plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 1/6/22
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 2 genes in this experiment
For carbon source Glycerol in Agrobacterium fabrum C58
For carbon source Glycerol across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamine biosynthesis I | 1 | 1 | 1 |
| ammonia assimilation cycle I | 2 | 2 | 1 |
| ammonia assimilation cycle II | 2 | 1 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 1 |
| superpathway of ammonia assimilation (plants) | 3 | 2 | 1 |
| L-aspartate degradation III (anaerobic) | 3 | 2 | 1 |
| L-aspartate degradation II (aerobic) | 3 | 2 | 1 |
| glycerol degradation I | 3 | 2 | 1 |
| glycerol and glycerophosphodiester degradation | 4 | 3 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 4 | 1 |
| L-arginine biosynthesis II (acetyl cycle) | 10 | 10 | 1 |