Experiment set10IT045 for Pseudomonas syringae pv. syringae B728a ΔmexB
KB with Novobiocin sodium salt 7.75 ug/ml
Group: stressMedia: KB + Novobiocin sodium salt (7.75 ug/ml)
Culturing: SyringaeB728a_mexBdelta_ML3, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 7/9/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate
Specific Phenotypes
For 12 genes in this experiment
For stress Novobiocin sodium salt in Pseudomonas syringae pv. syringae B728a ΔmexB
For stress Novobiocin sodium salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Bacterial Cell Division | 3 |
| Histidine Degradation | 1 |
| RNA processing and degradation, bacterial | 1 |
| Ribosome biogenesis bacterial | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fructose and mannose metabolism
- Pyrimidine metabolism
- Nucleotide sugars metabolism
- Aminosugars metabolism
- Ether lipid metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| GDP-mannose biosynthesis | 4 | 4 | 2 |
| D-mannose degradation I | 2 | 1 | 1 |
| D-mannose degradation II | 2 | 1 | 1 |
| pseudouridine degradation | 2 | 1 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 1 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 2 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 7 | 2 |
| lipoprotein posttranslational modification (Gram-negative bacteria) | 7 | 3 | 1 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |