Experiment set10IT044 for Pseudomonas putida KT2440

a-Ketoglutaric acid disodium salt hydrate carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + a-Ketoglutaric acid disodium salt hydrate (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 2.4 generations
By: Mitchell Thompson on 10/18/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 8 genes in this experiment

For carbon source a-Ketoglutaric acid disodium salt hydrate in Pseudomonas putida KT2440

For carbon source a-Ketoglutaric acid disodium salt hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Ammonia assimilation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.