Experiment set10IT033 for Pseudomonas putida KT2440

D-Glucose carbon source with 20AA_mix_minus_Thr

Group: carbon source
Media: MOPS minimal media_noCarbon + D-Glucose (10 mM) + 0.5X 20AA_mix_minus_Thr
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.7 generations
By: Mitchell Thompson on 10/1/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

20AA_mix_minus_Thr 0.5x includes: 0.25 mM L-Arginine, 0.25 mM L-Histidine, 0.25 mM L-Lysine, 0.25 mM L-Aspartic Acid, 0.25 mM L-Glutamic acid monopotassium salt monohydrate, 0.25 mM L-Serine, 0.25 mM L-Asparagine, 0.25 mM L-Glutamine, 0.25 mM L-Cysteine hydrochloride monohydrate, 0.25 mM Glycine, 0.25 mM L-Proline, 0.25 mM L-Alanine, 0.25 mM L-Valine, 0.25 mM L-Isoleucine, 0.25 mM L-Leucine, 0.25 mM L-Methionine, 0.25 mM L-Phenylalanine, 0.25 mM L-tyrosine disodium salt, 0.25 mM L-Tryptophan (final concentrations)

Specific Phenotypes

For 1 genes in this experiment

For carbon source D-Glucose in Pseudomonas putida KT2440

For carbon source D-Glucose across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.