Experiment set10IT032 for Escherichia coli BW25113

Compare to:

LB Aerobic with Oxytetracycline hydrochloride 0.0000003125 mM

200 most detrimental genes:

  gene name fitness t score description  
b0200 gmhB +2.8 4.6 hypothetical protein (NCBI) compare
b3052 rfaE +2.2 12.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2175 spr +2.1 9.7 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0464 acrR +2.0 15.6 DNA-binding transcriptional repressor (NCBI) compare
b0439 lon +2.0 10.6 DNA-binding ATP-dependent protease La (NCBI) compare
b3842 rfaH +1.9 11.4 transcriptional activator RfaH (NCBI) compare
b3631 rfaG +1.8 15.8 glucosyltransferase I (NCBI) compare
b1048 mdoG +1.8 10.4 glucan biosynthesis protein, periplasmic (NCBI) compare
b0222 lpcA +1.7 6.2 phosphoheptose isomerase (NCBI) compare
b3620 rfaF +1.6 9.0 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3205 yhbJ +1.6 6.4 hypothetical protein (NCBI) compare
b3630 rfaP +1.6 12.4 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3621 rfaC +1.5 9.2 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0929 ompF +1.5 10.2 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b1049 mdoH +1.5 11.0 glucosyltransferase MdoH (NCBI) compare
b4481 rffT +1.4 7.8 4-alpha-L-fucosyltransferase (NCBI) compare
b3791 rffA +1.4 8.4 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2912 ygfA +1.4 5.8 putative ligase (VIMSS) compare
b1530 marR +1.3 2.8 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0142 folK +1.3 1.2 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3619 rfaD +1.3 5.5 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1290 sapF +1.3 2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1628 rsxB +1.1 3.0 electron transport complex protein RnfB (NCBI) compare
b2183 rsuA +1.1 3.5 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b4552 yrhC +1.1 1.3 no description compare
b1236 galU +1.1 5.0 glucose-1-phosphate uridylyltransferase (NCBI) compare
b2951 yggS +1.1 4.8 predicted enzyme (NCBI) compare
b0926 ycbK +1.1 3.7 hypothetical protein (NCBI) compare
b1111 ycfQ +1.1 3.6 orf, hypothetical protein (VIMSS) compare
b3169 nusA +1.0 2.7 transcription elongation factor NusA (NCBI) compare
b3204 ptsN +1.0 4.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2356 yfdM +1.0 2.2 orf, hypothetical protein (VIMSS) compare
b4592 yccB +1.0 2.5 hypothetical protein (RefSeq) compare
b1613 manA +1.0 2.1 mannose-6-phosphate isomerase (VIMSS) compare
b0683 fur +1.0 1.5 ferric uptake regulator (NCBI) compare
b3790 rffC +1.0 3.8 TDP-fucosamine acetyltransferase (RefSeq) compare
b3916 pfkA +0.9 2.8 6-phosphofructokinase (NCBI) compare
b1412 azoR +0.9 1.7 acyl carrier protein phosphodiesterase (NCBI) compare
b2947 gshB +0.9 4.0 glutathione synthetase (NCBI) compare
b0965 b0965 +0.9 2.5 orf, hypothetical protein (VIMSS) compare
b0418 pgpA +0.9 4.0 phosphatidylglycerophosphatase A (NCBI) compare
b1217 chaB +0.9 1.0 cation transport regulator (NCBI) compare
b1984 asnW +0.9 1.6 tRNA-Asn (NCBI) compare
b1676 pykF +0.9 3.7 pyruvate kinase (NCBI) compare
b1292 sapC +0.9 1.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3405 ompR +0.9 4.3 osmolarity response regulator (NCBI) compare
b1921 fliZ +0.8 2.5 orf, hypothetical protein (VIMSS) compare
b2749 ygbE +0.8 4.9 conserved inner membrane protein (NCBI) compare
b1584 speG +0.8 4.5 spermidine N1-acetyltransferase (NCBI) compare
b0641 rlpB +0.8 2.5 minor lipoprotein (NCBI) compare
b1411 ynbD +0.8 3.3 predicted phosphatase, inner membrane protein (NCBI) compare
b2450 yffS +0.8 1.8 CPZ-55 prophage; predicted protein (RefSeq) compare
b1034 ycdX +0.8 3.7 hypothetical protein (NCBI) compare
b0632 dacA +0.8 5.1 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b0920 ycbC +0.8 3.5 conserved inner membrane protein (NCBI) compare
b1632 rsxE +0.8 5.2 NADH-ubiquinone oxidoreductase (NCBI) compare
b1865 nudB +0.8 1.4 dATP pyrophosphohydrolase (NCBI) compare
b2058 wcaB +0.8 1.1 predicted acyl transferase (NCBI) compare
b4431 rprA +0.8 1.6 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b3860 dsbA +0.8 5.2 periplasmic protein disulfide isomerase I (NCBI) compare
b1949 fliQ +0.8 1.1 flagellar biosynthesis protein Q (NCBI) compare
b1627 rsxA +0.7 2.8 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b4578 insP +0.7 2.0 no description compare
b2593 yfiH +0.7 4.9 hypothetical protein (NCBI) compare
b1850 eda +0.7 2.0 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1594 dgsA +0.7 1.9 DNA-binding transcriptional repressor (NCBI) compare
b4418 sraB +0.7 1.1 unknown RNA (NCBI) compare
b2618 b2618 +0.7 2.1 orf, hypothetical protein (VIMSS) compare
b4045 yjbJ +0.7 2.0 predicted stress response protein (NCBI) compare
b4413 sokC +0.7 1.6 regulatory antisense RNA (NCBI) compare
b1604 ydgH +0.7 3.9 hypothetical protein (NCBI) compare
b0737 tolQ +0.7 1.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1890 motA +0.7 1.8 flagellar motor protein MotA (NCBI) compare
b3792 wzxE +0.7 4.4 O-antigen translocase (NCBI) compare
b1811 yoaH +0.7 2.6 hypothetical protein (NCBI) compare
b4041 plsB +0.7 1.8 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1251 yciI +0.7 2.2 orf, hypothetical protein (VIMSS) compare
b2501 ppk +0.7 4.6 polyphosphate kinase (NCBI) compare
b4151 frdD +0.7 2.5 fumarate reductase subunit D (NCBI) compare
b3794 rffM +0.7 3.8 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1253 yciA +0.7 2.5 predicted hydrolase (NCBI) compare
b2340 sixA +0.7 2.9 phosphohistidine phosphatase (NCBI) compare
b2432 yfeY +0.7 1.4 hypothetical protein (NCBI) compare
b1684 sufA +0.7 1.4 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b2617 smpA +0.7 1.7 small membrane protein A (VIMSS) compare
b1667 ydhR +0.7 0.9 hypothetical protein (NCBI) compare
b0974 hyaC +0.6 2.2 hydrogenase 1, b-type cytochrome subunit (NCBI) compare
b1312 ycjP +0.6 3.5 predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3366 nirD +0.6 2.0 nitrite reductase small subunit (NCBI) compare
b1699 ydiS +0.6 2.1 predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI) compare
b2570 rseC +0.6 2.8 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b1582 ynfA +0.6 1.8 hypothetical protein (NCBI) compare
b1629 rsxC +0.6 3.3 electron transport complex protein RnfC (NCBI) compare
b2350 yfdG +0.6 3.8 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b1630 rsxD +0.6 2.7 electron transport complex protein RnfD (NCBI) compare
b3890 yiiF +0.6 1.6 orf, hypothetical protein (VIMSS) compare
b1295 ymjA +0.6 1.8 hypothetical protein (NCBI) compare
b2024 hisA +0.6 1.3 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b2808 gcvA +0.6 2.2 DNA-binding transcriptional dual regulator (NCBI) compare
b1355 b1355 +0.6 2.5 orf, hypothetical protein (VIMSS) compare
b1183 umuD +0.6 1.5 DNA polymerase V, subunit D (NCBI) compare
b0407 yajC +0.6 1.7 preprotein translocase subunit YajC (NCBI) compare
b1609 rstB +0.6 2.2 sensory histidine kinase in two-component regulatory system with RstA (NCBI) compare
b1161 ycgX +0.6 0.7 hypothetical protein (NCBI) compare
b1424 mdoD +0.6 3.1 glucan biosynthesis protein, periplasmic (RefSeq) compare
b3611 yibN +0.6 2.8 predicted rhodanese-related sulfurtransferase (NCBI) compare
b4545 ypdJ +0.6 1.3 no description compare
b2343 b2343 +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b4373 rimI +0.6 1.6 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b0191 yaeJ +0.6 2.5 hypothetical protein (NCBI) compare
b3838 tatB +0.6 3.4 sec-independent translocase (NCBI) compare
b1289 ycjD +0.6 1.5 hypothetical protein (NCBI) compare
b1633 nth +0.6 2.3 DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI) compare
b2904 gcvH +0.6 1.7 glycine cleavage system protein H (NCBI) compare
b2768 ygcP +0.6 1.8 predicted anti-terminator regulatory protein (NCBI) compare
b1647 ydhF +0.6 1.5 predicted oxidoreductase (NCBI) compare
b2187 yejL +0.5 1.7 hypothetical protein (NCBI) compare
b4215 ytfI +0.5 0.9 hypothetical protein (NCBI) compare
b0263 afuB +0.5 1.7 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b3068 ygjF +0.5 2.0 G/U mismatch-specific DNA glycosylase (NCBI) compare
b1487 ddpA +0.5 2.5 D-ala-D-a la transporter subunit (NCBI) compare
b0393 rdgC +0.5 3.8 recombination associated protein (NCBI) compare
b1305 pspB +0.5 1.7 phage shock protein B (NCBI) compare
b2236 yfaE +0.5 2.0 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b0961 yccF +0.5 2.5 conserved inner membrane protein (NCBI) compare
b2686 emrB +0.5 3.4 multidrug efflux system protein (NCBI) compare
b1842 holE +0.5 0.9 DNA polymerase III, theta subunit (NCBI) compare
b0889 lrp +0.5 1.5 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2784 relA +0.5 3.6 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b2531 iscR +0.5 0.8 DNA-binding transcriptional repressor (NCBI) compare
b0015 dnaJ +0.5 3.3 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1336 ydaH +0.5 2.3 putative pump protein (transport) (VIMSS) compare
b2940 yqgC +0.5 1.8 hypothetical protein (NCBI) compare
b2419 yfeK +0.5 1.0 hypothetical protein (NCBI) compare
b1349 recT +0.5 2.8 Rac prophage; recombination and repair protein (NCBI) compare
b1353 sieB +0.5 2.4 phage superinfection exclusion protein (VIMSS) compare
b4451 ryhB +0.5 0.7 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2708 gutQ +0.5 2.7 orf, hypothetical protein (VIMSS) compare
b0082 mraW +0.5 1.6 S-adenosyl-methyltransferase (NCBI) compare
b4692 oweE +0.5 1.3 no description compare
b3250 mreC +0.5 0.6 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b1060 yceP +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b3133 agaV +0.5 2.4 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b0798 ybiA +0.5 1.4 hypothetical protein (NCBI) compare
b2910 zapA +0.5 1.8 protein that localizes to the cytokinetic ring (NCBI) compare
b1419 ydcA +0.5 2.1 hypothetical protein (NCBI) compare
b1608 rstA +0.5 1.9 DNA-binding response regulator in two-component regulatory system with RstB (RefSeq) compare
b1446 ydcY +0.5 1.1 hypothetical protein (NCBI) compare
b4006 purH +0.5 3.8 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b4397 creA +0.5 1.6 hypothetical protein (NCBI) compare
b0504 ybbS +0.5 1.7 DNA-binding transcriptional activator of the allD operon (NCBI) compare
b3632 rfaQ +0.5 4.3 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2516 yfgA +0.5 2.4 hypothetical protein (NCBI) compare
b1603 pntA +0.5 1.6 NAD(P) transhydrogenase subunit alpha (NCBI) compare
b1095 fabF +0.5 2.8 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b4301 sgcE +0.5 2.9 ribulose-phosphate 3-epimerase (NCBI) compare
b2685 emrA +0.5 3.3 multidrug efflux system (NCBI) compare
b1063 yceB +0.5 1.3 predicted lipoprotein (NCBI) compare
b2905 gcvT +0.5 2.6 glycine cleavage system aminomethyltransferase T (NCBI) compare
b1901 araF +0.5 2.0 L-arabinose transporter subunit (NCBI) compare
b1618 uidR +0.5 1.1 DNA-binding transcriptional repressor (NCBI) compare
b0088 murD +0.5 0.7 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b2421 cysM +0.5 1.4 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b1248 b1248 +0.5 1.0 orf, hypothetical protein (VIMSS) compare
b0143 pcnB +0.5 2.4 poly(A) polymerase I (VIMSS) compare
b1504 ydeS +0.5 2.1 predicted fimbrial-like adhesin protein (NCBI) compare
b4515 ybgT +0.5 1.0 hypothetical protein (NCBI) compare
b0158 btuF +0.5 1.9 vitamin B12-transporter protein BtuF (NCBI) compare
b4093 phnO +0.5 2.4 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3940 metL +0.5 3.4 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b3026 qseC +0.5 1.4 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b4636 ybfI +0.5 2.4 no description compare
b4538 yoeF +0.5 0.7 hypothetical protein (RefSeq) compare
b0864 artP +0.5 1.6 arginine transporter subunit (NCBI) compare
b4460 araH +0.4 0.9 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b2346 vacJ +0.4 2.4 predicted lipoprotein (NCBI) compare
b1595 ynfL +0.4 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1524 yneH +0.4 0.5 predicted glutaminase (NCBI) compare
b2262 menB +0.4 1.7 naphthoate synthase (NCBI) compare
b0211 mltD +0.4 2.8 predicted membrane-bound lytic murein transglycosylase D (NCBI) compare
b2377 yfdY +0.4 1.4 predicted inner membrane protein (NCBI) compare
b1812 pabB +0.4 2.1 para-aminobenzoate synthase component I (NCBI) compare
b3761 trpT +0.4 0.9 tRNA-Trp (NCBI) compare
b2700 ygaD +0.4 2.1 competence damage-inducible protein A (NCBI) compare
b2659 b2659 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b3378 yhfU +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b3627 rfaI +0.4 2.9 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2081 yegQ +0.4 2.5 predicted peptidase (NCBI) compare
b1514 lsrC +0.4 0.7 AI2 transporter (NCBI) compare
b0782 moaB +0.4 1.0 molybdopterin biosynthesis protein B (NCBI) compare
b3194 yrbE +0.4 3.3 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3120 yhaB +0.4 0.7 orf, hypothetical protein (VIMSS) compare
b0456 ybaA +0.4 1.6 hypothetical protein (NCBI) compare
b0946 ycbW +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b2688 gshA +0.4 2.6 glutamate--cysteine ligase (NCBI) compare
b4438 cyaR +0.4 0.8 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1038 csgF +0.4 1.2 predicted transport protein (NCBI) compare
b3293 yhdN +0.4 1.2 hypothetical protein (NCBI) compare
b0157 yadS +0.4 2.2 conserved inner membrane protein (NCBI) compare
b0209 yafD +0.4 2.4 hypothetical protein (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Oxytetracycline hydrochloride across organisms