Experiment set10IT030 for Escherichia coli BW25113

Compare to:

LB Aerobic with Spiramycin 0.0000625 mM

200 most important genes:

  gene name fitness t score description  
b0462 acrB -5.7 -11.6 multidrug efflux system protein (NCBI) compare
b0463 acrA -5.6 -7.7 multidrug efflux system (NCBI) compare
b2188 yejM -5.2 -7.1 predicted hydrolase, inner membrane (NCBI) compare
b2512 yfgL -4.7 -4.5 protein assembly complex, lipoprotein component (NCBI) compare
b3035 tolC -4.6 -4.5 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b2494 yfgC -4.6 -8.2 predicted peptidase (NCBI) compare
b1677 lpp -4.3 -2.9 murein lipoprotein (NCBI) compare
b3842 rfaH -4.2 -2.9 transcriptional activator RfaH (NCBI) compare
b1765 ydjA -3.7 -1.9 predicted oxidoreductase (NCBI) compare
b3199 yrbK -3.7 -3.1 hypothetical protein (NCBI) compare
b3163 nlpI -3.3 -5.0 hypothetical protein (NCBI) compare
b3632 rfaQ -3.2 -16.1 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1830 prc -3.2 -4.4 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3631 rfaG -3.2 -6.1 glucosyltransferase I (NCBI) compare
b0990 cspG -3.2 -2.1 DNA-binding transcriptional regulator (NCBI) compare
b3052 rfaE -3.0 -4.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0677 nagA -3.0 -6.5 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b0222 lpcA -2.9 -2.8 phosphoheptose isomerase (NCBI) compare
b0053 surA -2.9 -2.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3620 rfaF -2.9 -5.2 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3630 rfaP -2.9 -2.8 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1361 ydaW -2.9 -1.4 orf, hypothetical protein (VIMSS) compare
b3198 kdsC -2.8 -4.6 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b1236 galU -2.8 -2.7 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0145 dksA -2.8 -3.2 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3600 mtlD -2.8 -12.5 mannitol-1-phosphate 5-dehydrogenase (NCBI) compare
b1164 ycgZ -2.7 -1.8 hypothetical protein (NCBI) compare
b1346 ydaQ -2.7 -1.8 orf, hypothetical protein (VIMSS) compare
b0741 pal -2.7 -3.6 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0957 ompA -2.6 -3.5 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b0740 tolB -2.6 -3.5 translocation protein TolB precursor (NCBI) compare
b3621 rfaC -2.6 -3.5 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0605 ahpC -2.6 -4.8 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b0737 tolQ -2.5 -1.7 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2501 ppk -2.5 -9.7 polyphosphate kinase (NCBI) compare
b0739 tolA -2.5 -1.7 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2642 yfjW -2.5 -1.6 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1855 lpxM -2.4 -6.2 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b0683 fur -2.4 -1.6 ferric uptake regulator (NCBI) compare
b2017 yefM -2.3 -2.7 orf, hypothetical protein (VIMSS) compare
b1831 proQ -2.3 -5.3 putative solute/DNA competence effector (NCBI) compare
b2064 asmA -2.3 -4.4 predicted assembly protein (NCBI) compare
b1291 sapD -2.3 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4480 hdfR -2.2 -5.2 transcriptional regulator HdfR (NCBI) compare
b0688 pgm -2.2 -3.2 phosphoglucomutase (NCBI) compare
b3962 udhA -2.2 -6.2 putative oxidoreductase (VIMSS) compare
b4163 glyV -2.2 -2.1 tRNA-Gly (NCBI) compare
b3619 rfaD -2.2 -2.9 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b4701 sokX -2.2 -1.4 sok-related sRNA, function unknown (RefSeq) compare
b0730 mngR -2.1 -1.4 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1863 ruvC -2.1 -1.9 Holliday junction resolvase (NCBI) compare
b3764 yifE -2.1 -5.5 hypothetical protein (NCBI) compare
b0630 lipB -2.1 -1.4 protein of lipoate biosynthesis (VIMSS) compare
b2620 smpB -1.9 -1.8 SsrA-binding protein (NCBI) compare
b0142 folK -1.9 -0.9 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2617 smpA -1.9 -2.4 small membrane protein A (VIMSS) compare
b3229 sspA -1.8 -2.1 stringent starvation protein A (NCBI) compare
b1882 cheY -1.8 -0.9 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3341 rpsG -1.8 -1.7 30S ribosomal protein S7 (NCBI) compare
b2168 fruK -1.8 -1.6 1-phosphofructokinase (NCBI) compare
b4631 ykgQ -1.7 -2.3 no description compare
b3026 qseC -1.7 -1.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b3183 obgE -1.7 -1.1 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1183 umuD -1.6 -2.4 DNA polymerase V, subunit D (NCBI) compare
b0088 murD -1.6 -2.1 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b0623 cspE -1.6 -2.3 cold shock protein E (NCBI) compare
b1476 fdnI -1.6 -1.3 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1192 ldcA -1.6 -5.1 L,D-carboxypeptidase A (NCBI) compare
b3839 tatC -1.5 -5.6 TatABCE protein translocation system subunit (NCBI) compare
b0706 ybfD -1.5 -1.4 hypothetical protein (NCBI) compare
b0738 tolR -1.5 -1.7 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3426 glpD -1.5 -3.0 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b3961 oxyR -1.5 -8.0 DNA-binding transcriptional dual regulator (NCBI) compare
b3021 ygiT -1.5 -2.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3628 rfaB -1.5 -6.0 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1524 yneH -1.4 -1.0 predicted glutaminase (NCBI) compare
b0437 clpP -1.4 -4.8 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b4220 ytfM -1.4 -9.3 predicted outer membrane protein and surface antigen (NCBI) compare
b1942 fliJ -1.4 -1.8 flagellar biosynthesis chaperone (NCBI) compare
b1984 asnW -1.4 -1.4 tRNA-Asn (NCBI) compare
b4221 ytfN -1.4 -10.8 hypothetical protein (NCBI) compare
b1109 ndh -1.4 -3.5 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b2363 yfdT -1.4 -2.4 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1961 dcm -1.4 -1.8 DNA cytosine methylase (NCBI) compare
b4025 pgi -1.4 -6.4 glucose-6-phosphate isomerase (NCBI) compare
b3237 argR -1.4 -3.6 arginine repressor (NCBI) compare
b3082 ygjM -1.4 -1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1848 yebG -1.3 -1.6 conserved protein regulated by LexA (NCBI) compare
b0014 dnaK -1.3 -1.7 molecular chaperone DnaK (NCBI) compare
b1290 sapF -1.3 -1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1332 ynaJ -1.3 -0.8 predicted inner membrane protein (NCBI) compare
b1600 mdtJ -1.3 -1.5 multidrug efflux system transporter (NCBI) compare
b4520 ymgF -1.3 -1.6 hypothetical protein (NCBI) compare
b3250 mreC -1.3 -2.0 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b1259 yciG -1.3 -1.1 orf, hypothetical protein (VIMSS) compare
b3627 rfaI -1.3 -6.0 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b0767 ybhE -1.3 -3.0 6-phosphogluconolactonase (NCBI) compare
b0617 citD -1.3 -2.6 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b4676 yoaK -1.3 -0.8 expressed protein (RefSeq) compare
b1658 purR -1.3 -4.6 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0726 sucA -1.3 -3.4 alpha-ketoglutarate decarboxylase (NCBI) compare
b2553 glnB -1.3 -3.7 regulatory protein P-II for glutamine synthetase (NCBI) compare
b1249 cls -1.3 -5.9 cardiolipin synthetase (NCBI) compare
b2567 rnc -1.3 -0.8 ribonuclease III (NCBI) compare
b1969 yedW -1.3 -2.9 putative 2-component transcriptional regulator (VIMSS) compare
b4503 yafF -1.2 -0.8 no description compare
b3469 zntA -1.2 -5.1 zinc, cobalt and lead efflux system (NCBI) compare
b4509 ylcG -1.2 -1.5 DLP12 prophage; predicted protein (NCBI) compare
b4698 mgrR -1.2 -2.8 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b4201 priB -1.2 -2.2 primosomal replication protein N (NCBI) compare
b3644 yicC -1.2 -4.7 hypothetical protein (NCBI) compare
b1506 yneL -1.2 -0.8 predicted transcriptional regulator (NCBI) compare
b3414 gntY -1.2 -4.3 predicted gluconate transport associated protein (NCBI) compare
b0440 hupB -1.2 -3.4 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b2894 xerD -1.2 -0.7 site-specific tyrosine recombinase XerD (NCBI) compare
b2935 tktA -1.2 -0.8 transketolase 1, thiamin-binding (NCBI) compare
b0499 ylbH -1.2 -2.7 conserved protein, rhs-like (RefSeq) compare
b2236 yfaE -1.2 -2.9 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b0436 tig -1.2 -5.5 trigger factor (NCBI) compare
b1726 yniB -1.1 -2.2 predicted inner membrane protein (NCBI) compare
b1730 ydjO -1.1 -0.7 predicted protein (RefSeq) compare
b2252 ais -1.1 -2.9 hypothetical protein (NCBI) compare
b4202 rpsR -1.1 -0.7 30S ribosomal protein S18 (NCBI) compare
b2820 recB -1.1 -0.7 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0058 rluA -1.1 -4.8 pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32) (NCBI) compare
b3204 ptsN -1.1 -3.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b0232 yafN -1.1 -0.7 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3613 yibP -1.1 -0.7 putative membrane protein (VIMSS) compare
b3728 pstS -1.1 -1.0 phosphate transporter subunit (NCBI) compare
b2506 yfgI -1.1 -2.0 hypothetical protein (NCBI) compare
b2808 gcvA -1.1 -2.6 DNA-binding transcriptional dual regulator (NCBI) compare
b3484 yhhI -1.1 -2.0 predicted transposase (NCBI) compare
b4375 prfC -1.1 -1.7 peptide chain release factor 3 (NCBI) compare
b3778 rep -1.1 -4.5 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1104 ycfL -1.1 -1.7 hypothetical protein (NCBI) compare
b2330 yfcB -1.0 -1.3 putative adenine-specific methylase (VIMSS) compare
b1824 yobF -1.0 -1.9 hypothetical protein (NCBI) compare
b1742 b1742 -1.0 -2.5 orf, hypothetical protein (VIMSS) compare
b0082 mraW -1.0 -2.2 S-adenosyl-methyltransferase (NCBI) compare
b2895 fldB -1.0 -2.3 flavodoxin 2 (NCBI) compare
b1861 ruvA -1.0 -1.0 Holliday junction DNA helicase motor protein (NCBI) compare
b3446 yrhB -1.0 -3.2 hypothetical protein (NCBI) compare
b2774 ygcW -1.0 -2.0 putative oxidoreductase (VIMSS) compare
b0225 yafQ -1.0 -2.6 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b4179 vacB -1.0 -3.4 putative enzyme (VIMSS) compare
b3916 pfkA -1.0 -1.3 6-phosphofructokinase (NCBI) compare
b2528 iscA -1.0 -1.4 iron-sulfur cluster assembly protein (NCBI) compare
b3726 pstA -1.0 -2.9 phosphate transporter subunit (NCBI) compare
b4505 insX -1.0 -1.4 predicted IS protein (RefSeq) compare
b4541 yehK -1.0 -2.7 hypothetical protein (NCBI) compare
b3389 aroB -1.0 -5.5 3-dehydroquinate synthase (NCBI) compare
b3443 yrhA -1.0 -1.2 orf, hypothetical protein (VIMSS) compare
b4568 ytjA -1.0 -1.6 predicted protein (RefSeq) compare
b1579 intQ -1.0 -1.4 Qin prophage; predicted defective integrase (RefSeq) compare
b2369 evgA -0.9 -1.9 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b0149 mrcB -0.9 -6.8 penicillin-binding protein 1b (NCBI) compare
b2435 amiA -0.9 -3.0 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1753 ynjA -0.9 -2.0 hypothetical protein (NCBI) compare
b2007 yeeX -0.9 -2.4 conserved protein (RefSeq) compare
b4640 yoeG -0.9 -1.3 no description compare
b2569 lepA -0.9 -2.3 GTP-binding protein LepA (NCBI) compare
b1344 ydaO -0.9 -3.1 predicted C32 tRNA thiolase (NCBI) compare
b0547 ybcN -0.9 -1.8 DLP12 prophage; predicted protein (NCBI) compare
b1269 rluB -0.9 -2.4 23S rRNA pseudouridylate synthase (NCBI) compare
b2375 yfdX -0.9 -1.8 hypothetical protein (NCBI) compare
b4146 yjeK -0.9 -0.8 predicted lysine aminomutase (NCBI) compare
b4608 ryfC -0.9 -2.5 Novel sRNA, function unknown (RefSeq) compare
b2071 yegJ -0.9 -1.1 hypothetical protein (NCBI) compare
b3811 xerC -0.9 -0.6 site-specific tyrosine recombinase XerC (NCBI) compare
b0724 sdhB -0.9 -4.0 succinate dehydrogenase, FeS subunit (NCBI) compare
b1502 ydeQ -0.9 -0.8 predicted fimbrial-like adhesin protein (NCBI) compare
b1951 rcsA -0.9 -0.8 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b4383 deoB -0.9 -5.0 phosphopentomutase (NCBI) compare
b4494 arpB -0.9 -0.6 no description compare
b1973 yodA -0.9 -2.6 conserved metal-binding protein (NCBI) compare
b4239 treC -0.9 -1.3 trehalose-6-P hydrolase (NCBI) compare
b1573 ydfC -0.9 -1.2 Qin prophage; predicted protein (RefSeq) compare
b4313 fimE -0.9 -4.4 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b4546 ypeB -0.9 -0.9 hypothetical protein (NCBI) compare
b0722 sdhD -0.8 -2.9 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b0263 afuB -0.8 -2.0 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b1123 potD -0.8 -3.1 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b2498 upp -0.8 -1.6 uracil phosphoribosyltransferase (VIMSS) compare
b3290 trkA -0.8 -0.8 potassium transporter peripheral membrane component (NCBI) compare
b3629 rfaS -0.8 -1.4 lipopolysaccharide core biosynthesis protein (NCBI) compare
b4260 pepA -0.8 -5.5 leucyl aminopeptidase (NCBI) compare
b3650 spoT -0.8 -3.4 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b4000 hupA -0.8 -2.1 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1159 mcrA -0.8 -0.5 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b1309 ycjM -0.8 -2.1 putative polysaccharide hydrolase (VIMSS) compare
b4515 ybgT -0.8 -1.2 hypothetical protein (NCBI) compare
b1108 ycfP -0.8 -2.2 orf, hypothetical protein (VIMSS) compare
b3868 glnG -0.8 -2.5 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b0469 apt -0.8 -3.8 adenine phosphoribosyltransferase (NCBI) compare
b3247 cafA -0.8 -4.7 bundles of cytoplasmic filaments (VIMSS) compare
b4579 yaiX -0.8 -2.7 no description compare
b4588 ylcH -0.8 -2.6 hypothetical protein, DLP12 prophage (RefSeq) compare
b4622 ytcA -0.8 -3.1 hypothetical protein (RefSeq) compare
b2215 ompC -0.8 -4.6 outer membrane porin protein C (NCBI) compare
b2144 sanA -0.8 -5.1 hypothetical protein (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms