Experiment set10IT030 for Escherichia coli BW25113

LB Aerobic with Spiramycin 0.0000625 mM

Group: stress
Media: LB + Spiramycin (6.25E-02 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 6 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms

SEED Subsystems

Subsystem	#Specific
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Mannitol Utilization	1
Proteasome bacterial	1
Proteolysis in bacteria, ATP-dependent	1
Purine conversions	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
mannitol degradation I	1	1	1
guanine and guanosine salvage II	2	2	1
guanine and guanosine salvage I	2	2	1
xanthine and xanthosine salvage	2	2	1
adenine salvage	3	3	1
superpathway of guanine and guanosine salvage	3	3	1
adenine and adenosine salvage III	4	4	1
mannitol cycle	5	4	1
superpathway of purine nucleotide salvage	14	13	1
superpathway of hexitol degradation (bacteria)	18	18	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	19	1