Experiment set10IT028 for Escherichia coli BW25113

LB Aerobic with Phenazine ethosulfate 0.0000625 mM

Group: stress
Media: LB + Phenazine ethosulfate (6.25E-02 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 41 genes in this experiment

For stress Phenazine ethosulfate in Escherichia coli BW25113

For stress Phenazine ethosulfate across organisms

SEED Subsystems

Subsystem	#Specific
Transport of Iron	3
Iron acquisition in Vibrio	2
Lactate utilization	2
Oxidative stress	2
Alanine biosynthesis	1
Biogenesis of cytochrome c oxidases	1
Campylobacter Iron Metabolism	1
Capsular heptose biosynthesis	1
D-allose utilization	1
Experimental tye	1
Flavodoxin	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Glycine Biosynthesis	1
Glycine and Serine Utilization	1
Heme and Siroheme Biosynthesis	1
LOS core oligosaccharide biosynthesis	1
Lactose utilization	1
Lipid A modifications	1
Murein hydrolase regulation and cell death	1
NAD and NADP cofactor biosynthesis global	1
NAD regulation	1
Nitric oxide synthase	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
PnuC-like transporters	1
Staphylococcal phi-Mu50B-like prophages	1
Sulfur oxidation	1
Thioredoxin-disulfide reductase	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Selenoamino acid metabolism
• Nicotinate and nicotinamide metabolism
• Pentose and glucuronate interconversions
• Galactose metabolism
• Photosynthesis
• Glycine, serine and threonine metabolism
• Tryptophan metabolism
• Aminophosphonate metabolism
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Inositol phosphate metabolism
• Glycerophospholipid metabolism
• Sphingolipid metabolism
• Thiamine metabolism
• Folate biosynthesis
• Porphyrin and chlorophyll metabolism
• Biosynthesis of ansamycins

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NAD salvage pathway IV (from nicotinamide riboside)	2	2	2
L-cysteine degradation IV	1	1	1
cis-cyclopropane fatty acid (CFA) biosynthesis	1	1	1
L-alanine biosynthesis III	1	1	1
S-methyl-5'-thioadenosine degradation II	1	1	1
adenine and adenosine salvage III	4	4	3
adenine and adenosine salvage V	3	3	2
purine ribonucleosides degradation	6	6	3
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
superoxide radicals degradation	2	2	1
adenine and adenosine salvage I	2	2	1
polyphosphate metabolism	2	2	1
L-threonine degradation II	2	2	1
NAD biosynthesis from nicotinamide	2	1	1
cytidylyl molybdenum cofactor sulfurylation	2	1	1
sterculate biosynthesis	2	1	1
adenosine nucleotides degradation II	5	5	2
cardiolipin biosynthesis I	3	3	1
cardiolipin biosynthesis II	3	3	1
bis(guanylyl molybdopterin) cofactor sulfurylation	3	1	1
thiazole component of thiamine diphosphate biosynthesis II	7	5	2
heme b biosynthesis I (aerobic)	4	4	1
superpathway of L-alanine biosynthesis	4	4	1
reactive oxygen species degradation	4	4	1
heme b biosynthesis V (aerobic)	4	4	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane)	4	1	1
photosynthesis light reactions	4	1	1
tRNA-uridine 2-thiolation (mammalian mitochondria)	4	1	1
GDP-D-glycero-α-D-manno-heptose biosynthesis	4	1	1
tRNA-uridine 2-thiolation (yeast mitochondria)	4	1	1
uracil degradation III	5	5	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
ADP-L-glycero-β-D-manno-heptose biosynthesis	5	5	1
[2Fe-2S] iron-sulfur cluster biosynthesis	10	4	2
tRNA-uridine 2-thiolation (thermophilic bacteria)	5	1	1
purine nucleotides degradation II (aerobic)	11	11	2
superpathway of thiamine diphosphate biosynthesis II	11	9	2
molybdopterin biosynthesis	6	6	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	6	1
ppGpp metabolism	6	6	1
thiazole component of thiamine diphosphate biosynthesis I	6	6	1
superpathway of photosynthetic hydrogen production	6	3	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	2
ethene biosynthesis III (microbes)	7	6	1
superpathway of NAD biosynthesis in eukaryotes	14	7	2
superpathway of heme b biosynthesis from glycine	8	7	1
tRNA-uridine 2-thiolation (cytoplasmic)	8	1	1
3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent)	9	3	1
3,8-divinyl-chlorophyllide a biosynthesis III (aerobic, light independent)	9	3	1
superpathway of thiamine diphosphate biosynthesis I	10	10	1
superpathway of heme b biosynthesis from glutamate	10	10	1
nucleoside and nucleotide degradation (archaea)	10	4	1
tRNA-uridine 2-thiolation and selenation (bacteria)	11	7	1
L-methionine salvage cycle III	11	5	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	1
superpathway of purine nucleotide salvage	14	13	1
oxygenic photosynthesis	17	11	1
superpathway of L-threonine metabolism	18	16	1
ethene biosynthesis V (engineered)	25	18	1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered)	26	19	1
1-butanol autotrophic biosynthesis (engineered)	27	19	1
mycolate biosynthesis	205	25	3
superpathway of mycolate biosynthesis	239	26	3