Experiment set10IT024 for Pseudomonas putida KT2440
D-Glucose carbon source with 20AA_mix_minus_Gly
Group: carbon sourceMedia: MOPS minimal media_noCarbon + D-Glucose (10 mM) + 0.5X 20AA_mix_minus_Gly
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 2.4 generations
By: Mitchell Thompson on 10/1/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
20AA_mix_minus_Gly 0.5x includes: 0.25 mM L-Arginine, 0.25 mM L-Histidine, 0.25 mM L-Lysine, 0.25 mM L-Aspartic Acid, 0.25 mM L-Glutamic acid monopotassium salt monohydrate, 0.25 mM L-Serine, 0.25 mM L-Threonine, 0.25 mM L-Asparagine, 0.25 mM L-Glutamine, 0.25 mM L-Cysteine hydrochloride monohydrate, 0.25 mM L-Proline, 0.25 mM L-Alanine, 0.25 mM L-Valine, 0.25 mM L-Isoleucine, 0.25 mM L-Leucine, 0.25 mM L-Methionine, 0.25 mM L-Phenylalanine, 0.25 mM L-tyrosine disodium salt, 0.25 mM L-Tryptophan (final concentrations)
Specific Phenotypes
None in this experiment