Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| L-alanine biosynthesis III | 1 | 1 | 1 |
| long-chain fatty acid activation | 1 | 1 | 1 |
| mannitol degradation I | 1 | 1 | 1 |
| S-methyl-5'-thioadenosine degradation II | 1 | 1 | 1 |
| arginine dependent acid resistance | 1 | 1 | 1 |
| L-cysteine degradation IV | 1 | 1 | 1 |
| adenine and adenosine salvage III | 4 | 4 | 3 |
| adenine and adenosine salvage V | 3 | 3 | 2 |
| neolinustatin bioactivation | 3 | 2 | 2 |
| purine ribonucleosides degradation | 6 | 6 | 3 |
| putrescine biosynthesis I | 2 | 2 | 1 |
| di-trans,poly-cis-undecaprenyl phosphate biosynthesis | 2 | 2 | 1 |
| L-arginine degradation III (arginine decarboxylase/agmatinase pathway) | 2 | 2 | 1 |
| adenine and adenosine salvage I | 2 | 2 | 1 |
| linustatin bioactivation | 4 | 2 | 2 |
| lotaustralin degradation | 2 | 1 | 1 |
| linoleate biosynthesis II (animals) | 2 | 1 | 1 |
| linamarin degradation | 2 | 1 | 1 |
| cytidylyl molybdenum cofactor sulfurylation | 2 | 1 | 1 |
| γ-linolenate biosynthesis II (animals) | 2 | 1 | 1 |
| cinnamoyl-CoA biosynthesis | 2 | 1 | 1 |
| adenosine nucleotides degradation II | 5 | 5 | 2 |
| cardiolipin biosynthesis I | 3 | 3 | 1 |
| cardiolipin biosynthesis II | 3 | 3 | 1 |
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 1 |
| L-serine degradation | 3 | 3 | 1 |
| D-serine degradation | 3 | 3 | 1 |
| cellulose degradation II (fungi) | 3 | 2 | 1 |
| L-methionine degradation II | 3 | 2 | 1 |
| L-cysteine degradation II | 3 | 2 | 1 |
| 3-methyl-branched fatty acid α-oxidation | 6 | 3 | 2 |
| oleate biosynthesis I (plants) | 3 | 1 | 1 |
| L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) | 3 | 1 | 1 |
| alkane biosynthesis I | 3 | 1 | 1 |
| putrescine biosynthesis II | 3 | 1 | 1 |
| pectin degradation I | 3 | 1 | 1 |
| heptadecane biosynthesis | 3 | 1 | 1 |
| bis(guanylyl molybdopterin) cofactor sulfurylation | 3 | 1 | 1 |
| alkane biosynthesis II | 3 | 1 | 1 |
| peptidoglycan recycling II | 10 | 7 | 3 |
| thiazole component of thiamine diphosphate biosynthesis II | 7 | 5 | 2 |
| superpathway of L-alanine biosynthesis | 4 | 4 | 1 |
| cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas) | 4 | 3 | 1 |
| phytol degradation | 4 | 3 | 1 |
| superpathway of putrescine biosynthesis | 4 | 3 | 1 |
| spermidine biosynthesis III | 4 | 2 | 1 |
| phosphatidylcholine acyl editing | 4 | 2 | 1 |
| wax esters biosynthesis II | 4 | 1 | 1 |
| tRNA-uridine 2-thiolation (mammalian mitochondria) | 4 | 1 | 1 |
| tRNA-uridine 2-thiolation (yeast mitochondria) | 4 | 1 | 1 |
| long chain fatty acid ester synthesis (engineered) | 4 | 1 | 1 |
| pinosylvin metabolism | 4 | 1 | 1 |
| sporopollenin precursors biosynthesis | 18 | 5 | 4 |
| peptidoglycan recycling I | 14 | 14 | 3 |
| polyisoprenoid biosynthesis (E. coli) | 5 | 5 | 1 |
| 5,6-dehydrokavain biosynthesis (engineered) | 10 | 8 | 2 |
| mannitol cycle | 5 | 4 | 1 |
| pectin degradation II | 5 | 3 | 1 |
| [2Fe-2S] iron-sulfur cluster biosynthesis | 10 | 4 | 2 |
| sphingosine and sphingosine-1-phosphate metabolism | 10 | 4 | 2 |
| octane oxidation | 5 | 2 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 2 | 1 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 1 |
| coumarin biosynthesis (via 2-coumarate) | 5 | 2 | 1 |
| tRNA-uridine 2-thiolation (thermophilic bacteria) | 5 | 1 | 1 |
| purine nucleotides degradation II (aerobic) | 11 | 11 | 2 |
| superpathway of thiamine diphosphate biosynthesis II | 11 | 9 | 2 |
| L-threonine degradation I | 6 | 6 | 1 |
| molybdopterin biosynthesis | 6 | 6 | 1 |
| thiazole component of thiamine diphosphate biosynthesis I | 6 | 6 | 1 |
| stearate biosynthesis II (bacteria and plants) | 6 | 5 | 1 |
| L-methionine biosynthesis II | 6 | 5 | 1 |
| fatty acid salvage | 6 | 5 | 1 |
| stearate biosynthesis IV | 6 | 4 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 6 | 2 |
| 6-gingerol analog biosynthesis (engineered) | 6 | 2 | 1 |
| stearate biosynthesis I (animals) | 6 | 1 | 1 |
| α-tomatine degradation | 6 | 1 | 1 |
| superpathway of cardiolipin biosynthesis (bacteria) | 13 | 11 | 2 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 1 |
| lipoprotein posttranslational modification (Gram-negative bacteria) | 7 | 6 | 1 |
| glycine betaine degradation III | 7 | 4 | 1 |
| ceramide degradation by α-oxidation | 7 | 2 | 1 |
| icosapentaenoate biosynthesis III (8-desaturase, mammals) | 7 | 1 | 1 |
| icosapentaenoate biosynthesis II (6-desaturase, mammals) | 7 | 1 | 1 |
| capsaicin biosynthesis | 7 | 1 | 1 |
| arachidonate biosynthesis III (6-desaturase, mammals) | 7 | 1 | 1 |
| superpathway of polyamine biosynthesis I | 8 | 7 | 1 |
| superpathway of polyamine biosynthesis II | 8 | 4 | 1 |
| glycine betaine degradation I | 8 | 4 | 1 |
| L-mimosine degradation | 8 | 4 | 1 |
| glutathione-mediated detoxification I | 8 | 3 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 3 | 1 |
| ceramide and sphingolipid recycling and degradation (yeast) | 16 | 4 | 2 |
| tRNA-uridine 2-thiolation (cytoplasmic) | 8 | 1 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 4 | 1 |
| superpathway of thiamine diphosphate biosynthesis I | 10 | 10 | 1 |
| nucleoside and nucleotide degradation (archaea) | 10 | 4 | 1 |
| 3-phenylpropanoate degradation | 10 | 4 | 1 |
| suberin monomers biosynthesis | 20 | 3 | 2 |
| superpathway of fatty acid biosynthesis II (plant) | 43 | 38 | 4 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 11 | 1 |
| tRNA-uridine 2-thiolation and selenation (bacteria) | 11 | 7 | 1 |
| L-methionine salvage cycle III | 11 | 5 | 1 |
| mycobactin biosynthesis | 11 | 3 | 1 |
| superpathway of phospholipid biosynthesis III (E. coli) | 12 | 12 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 13 | 1 |
| superpathway of purine nucleotide salvage | 14 | 13 | 1 |
| hypoglycin biosynthesis | 14 | 4 | 1 |
| firefly bioluminescence | 14 | 2 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 1 |
| palmitate biosynthesis II (type II fatty acid synthase) | 31 | 29 | 2 |
| cutin biosynthesis | 16 | 2 | 1 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 16 | 1 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 1 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 18 | 1 |
| superpathway of L-threonine metabolism | 18 | 16 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 19 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 17 | 1 |
| superpathway of fatty acids biosynthesis (E. coli) | 53 | 51 | 2 |
| palmitate biosynthesis III | 29 | 21 | 1 |
| oleate β-oxidation | 35 | 32 | 1 |