Experiment set10IT010 for Pseudomonas putida KT2440
L-Histidine carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + L-Histidine (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 4.9 generations
By: Mitchell Thompson on 10/1/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 8 genes in this experiment
For carbon source L-Histidine in Pseudomonas putida KT2440
For carbon source L-Histidine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Histidine Degradation | 6 |
| Methionine Biosynthesis | 1 |
| One-carbon metabolism by tetrahydropterines | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Histidine metabolism
- Glyoxylate and dicarboxylate metabolism
- One carbon pool by folate
- Nitrogen metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-histidine degradation II | 5 | 5 | 5 |
| L-histidine degradation I | 4 | 4 | 4 |
| L-histidine degradation III | 6 | 4 | 3 |
| L-histidine degradation VI | 8 | 7 | 3 |
| formaldehyde oxidation VII (THF pathway) | 4 | 4 | 1 |
| folate transformations I | 13 | 9 | 1 |
| purine nucleobases degradation I (anaerobic) | 15 | 6 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 1 |