Experiment set10IT009 for Pseudomonas putida KT2440

L-Glutamine carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + L-Glutamine (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 4.9 generations
By: Mitchell Thompson on 10/1/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 11 genes in this experiment

For carbon source L-Glutamine in Pseudomonas putida KT2440

For carbon source L-Glutamine across organisms

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	1
Cysteine Biosynthesis	1
Mannose Metabolism	1
Pyrimidine utilization	1
Transport of Zinc	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Methionine metabolism
• Lysine biosynthesis
• Tyrosine metabolism
• Phenylalanine metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Novobiocin biosynthesis
• Nucleotide sugars metabolism
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
3-(4-hydroxyphenyl)pyruvate biosynthesis	1	1	1
L-phenylalanine biosynthesis III (cytosolic, plants)	2	2	1
D-mannose degradation II	2	1	1
L-tyrosine degradation II	2	1	1
D-mannose degradation I	2	1	1
atromentin biosynthesis	2	1	1
L-phenylalanine biosynthesis I	3	3	1
L-tyrosine biosynthesis I	3	3	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
L-phenylalanine degradation II (anaerobic)	3	2	1
L-tyrosine degradation IV (to 4-methylphenol)	3	1	1
mannitol biosynthesis	3	1	1
GDP-mannose biosynthesis	4	4	1
L-phenylalanine degradation III	4	2	1
L-tyrosine degradation III	4	2	1
mannitol degradation II	4	2	1
L-tyrosine degradation I	5	5	1
4-hydroxybenzoate biosynthesis I (eukaryotes)	5	2	1
superpathway of plastoquinol biosynthesis	5	2	1
1,5-anhydrofructose degradation	5	2	1
L-tyrosine degradation V (reductive Stickland reaction)	5	1	1
L-phenylalanine degradation VI (reductive Stickland reaction)	5	1	1
β-(1,4)-mannan degradation	7	2	1
superpathway of aromatic amino acid biosynthesis	18	18	2
L-phenylalanine degradation IV (mammalian, via side chain)	9	6	1
superpathway of L-tyrosine biosynthesis	10	10	1
superpathway of L-phenylalanine biosynthesis	10	10	1
rosmarinic acid biosynthesis I	10	2	1
colanic acid building blocks biosynthesis	11	9	1
(S)-reticuline biosynthesis I	11	3	1
tropane alkaloids biosynthesis	11	1	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	1
superpathway of rosmarinic acid biosynthesis	14	2	1
superpathway of hyoscyamine (atropine) and scopolamine biosynthesis	16	3	1
anaerobic aromatic compound degradation (Thauera aromatica)	27	4	1
superpathway of chorismate metabolism	59	42	2