Experiment set10IT008 for Pseudomonas putida KT2440
L-Glutamic acid monopotassium salt monohydrate carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + L-Glutamic acid monopotassium salt monohydrate (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 4.3 generations
By: Mitchell Thompson on 10/1/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 13 genes in this experiment
For carbon source L-Glutamic acid monopotassium salt monohydrate in Pseudomonas putida KT2440
For carbon source L-Glutamic acid monopotassium salt monohydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Beta-lactamase | 1 |
| Cysteine Biosynthesis | 1 |
| Glycerolipid and Glycerophospholipid Metabolism in Bacteria | 1 |
| Phosphate metabolism | 1 |
| Pyrimidine utilization | 1 |
| Tn552 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Penicillin and cephalosporin biosynthesis
- beta-Lactam resistance
- Glycerolipid metabolism
- Glycerophospholipid metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: