Experiment set10IT005 for Escherichia coli BW25113

Compare to:

LB Aerobic with Spiramycin 0.000125 mM

200 most detrimental genes:

  gene name fitness t score description  
b0464 acrR +4.4 18.8 DNA-binding transcriptional repressor (NCBI) compare
b0439 lon +3.7 16.2 DNA-binding ATP-dependent protease La (NCBI) compare
b3390 aroK +2.9 6.9 shikimate kinase I (NCBI) compare
b1530 marR +2.9 5.6 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0955 ycbZ +2.5 16.6 predicted peptidase (NCBI) compare
b1187 fadR +1.9 9.0 fatty acid metabolism regulator (NCBI) compare
b3493 pitA +1.9 12.9 phosphate transporter, low-affinity (NCBI) compare
b2517 yfgB +1.8 9.7 predicted enzyme (NCBI) conserved
b0912 ihfB +1.5 6.3 integration host factor subunit beta (NCBI) compare
b2518 ndk +1.5 4.3 nucleoside diphosphate kinase (NCBI) compare
b2830 nudH +1.5 2.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b0685 ybfE +1.4 5.8 orf, hypothetical protein (VIMSS) compare
b2318 truA +1.3 4.2 tRNA pseudouridine synthase A (NCBI) compare
b1563 relE +1.2 2.9 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b1217 chaB +1.2 1.6 cation transport regulator (NCBI) compare
b4685 yrbN +1.2 3.6 expressed protein (RefSeq) compare
b2937 speB +1.1 5.6 agmatinase (NCBI) compare
b4246 pyrL +1.1 3.2 pyrBI operon leader peptide (NCBI) compare
b2688 gshA +1.0 5.7 glutamate--cysteine ligase (NCBI) compare
b2259 pmrD +1.0 1.3 polymyxin resistance protein B (VIMSS) compare
b2618 b2618 +1.0 4.5 orf, hypothetical protein (VIMSS) compare
b4203 rplI +1.0 4.3 50S ribosomal protein L9 (NCBI) compare
b1805 fadD +1.0 6.8 acyl-CoA synthase (NCBI) compare
b1627 rsxA +1.0 3.2 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b2450 yffS +1.0 1.9 CPZ-55 prophage; predicted protein (RefSeq) compare
b2990 hybG +0.9 3.0 hydrogenase 2 accessory protein (NCBI) compare
b2058 wcaB +0.9 1.4 predicted acyl transferase (NCBI) compare
b1524 yneH +0.9 1.1 predicted glutaminase (NCBI) compare
b2570 rseC +0.9 3.5 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b1263 trpD +0.9 2.8 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b1632 rsxE +0.9 5.2 NADH-ubiquinone oxidoreductase (NCBI) compare
b1827 kdgR +0.9 3.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1346 ydaQ +0.9 1.4 orf, hypothetical protein (VIMSS) compare
b1628 rsxB +0.9 2.1 electron transport complex protein RnfB (NCBI) compare
b2947 gshB +0.8 3.6 glutathione synthetase (NCBI) compare
b1865 nudB +0.8 1.3 dATP pyrophosphohydrolase (NCBI) compare
b0247 ykfG +0.8 1.4 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b4063 soxR +0.8 3.2 DNA-binding transcriptional dual regulator, Fe-S center for redox-sensing (NCBI) compare
b2938 speA +0.8 5.5 arginine decarboxylase (NCBI) compare
b0926 ycbK +0.8 3.3 hypothetical protein (NCBI) compare
b3258 panF +0.8 5.5 sodium/pantothenate symporter (VIMSS) compare
b1138 ymfE +0.8 1.6 e14 prophage; predicted inner membrane protein (NCBI) compare
b1253 yciA +0.8 2.8 predicted hydrolase (NCBI) compare
b1765 ydjA +0.7 1.1 predicted oxidoreductase (NCBI) compare
b4570 lomR +0.7 1.6 no description compare
b0523 purE +0.7 3.0 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b2684 mprA +0.7 2.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2502 ppx +0.7 4.6 exopolyphosphatase (NCBI) compare
b2093 gatB +0.7 2.9 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b4532 yncN +0.7 1.3 orf, hypothetical protein (VIMSS) compare
b1883 cheB +0.7 3.3 chemotaxis-specific methylesterase (NCBI) compare
b1048 mdoG +0.7 3.5 glucan biosynthesis protein, periplasmic (NCBI) compare
b1049 mdoH +0.7 4.9 glucosyltransferase MdoH (NCBI) compare
b0495 ybbA +0.7 3.3 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3169 nusA +0.7 2.2 transcription elongation factor NusA (NCBI) compare
b1640 ydhH +0.7 2.8 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b1316 ycjT +0.7 1.8 predicted hydrolase (NCBI) compare
b0418 pgpA +0.7 3.5 phosphatidylglycerophosphatase A (NCBI) compare
b0125 hpt +0.7 3.5 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b1143 ymfI +0.7 1.1 orf, hypothetical protein (VIMSS) compare
b2904 gcvH +0.7 1.5 glycine cleavage system protein H (NCBI) compare
b4011 yjaA +0.6 2.5 hypothetical protein (NCBI) compare
b2214 yojL +0.6 3.2 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b0763 modA +0.6 3.2 molybdate transporter subunit (NCBI) compare
b0817 mntR +0.6 1.9 DNA-binding transcriptional regulator of mntH (NCBI) compare
b4458 oxyS +0.6 0.7 global regulatory RNA (NCBI) compare
b3555 yiaG +0.6 2.1 predicted transcriptional regulator (NCBI) compare
b4158 yjeO +0.6 1.8 conserved inner membrane protein (NCBI) compare
b1850 eda +0.6 1.0 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b3095 yqjA +0.6 4.1 conserved inner membrane protein (NCBI) compare
b1891 flhC +0.6 2.7 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b1581 rspA +0.6 2.2 predicted dehydratase (NCBI) compare
b4557 yidD +0.6 2.2 hypothetical protein (NCBI) compare
b1890 motA +0.6 2.6 flagellar motor protein MotA (NCBI) compare
b1752 ydjZ +0.6 1.5 conserved inner membrane protein (NCBI) compare
b1630 rsxD +0.6 3.1 electron transport complex protein RnfD (NCBI) compare
b1803 yeaX +0.6 1.9 predicted oxidoreductase (NCBI) compare
b4577 sgrS +0.6 1.5 small antisense RNA (NCBI) compare
b2319 usg +0.6 2.0 hypothetical protein (NCBI) compare
b2134 pbpG +0.6 3.5 penicillin-binding protein 7 (VIMSS) compare
b3863 polA +0.6 0.5 DNA polymerase I (NCBI) compare
b1424 mdoD +0.6 3.1 glucan biosynthesis protein, periplasmic (RefSeq) compare
b4589 ylcI +0.6 1.4 hypothetical protein (RefSeq) compare
b1915 yecF +0.6 2.9 hypothetical protein (NCBI) compare
b1148 ymfM +0.6 1.6 e14 prophage; predicted protein (NCBI) compare
b3206 npr +0.6 2.2 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1296 ycjJ +0.6 1.7 putative amino acid/amine transport protein (VIMSS) compare
b3221 yhcH +0.6 2.4 hypothetical protein (NCBI) compare
b1606 folM +0.6 2.9 short chain dehydrogenase (NCBI) compare
b2426 ucpA +0.6 0.9 putative oxidoreductase (VIMSS) compare
b4215 ytfI +0.5 0.9 hypothetical protein (NCBI) compare
b1751 ydjY +0.5 2.1 orf, hypothetical protein (VIMSS) compare
b4436 ryeC +0.5 0.6 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b1671 ydhX +0.5 1.6 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b2597 yfiA +0.5 1.3 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b1729 ydjN +0.5 1.8 predicted transporter (NCBI) compare
b2263 yfbB +0.5 1.2 predicted peptidase (NCBI) compare
b4178 yjeB +0.5 2.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b2951 yggS +0.5 2.4 predicted enzyme (NCBI) compare
b1569 dicC +0.5 2.0 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b0753 ybgS +0.5 2.0 hypothetical protein (NCBI) compare
b1853 yebK +0.5 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b3070 yqjH +0.5 3.1 predicted siderophore interacting protein (NCBI) compare
b1557 cspB +0.5 0.8 Qin prophage; cold shock protein (NCBI) compare
b1179 ycgL +0.5 1.4 conserved protein (NCBI) compare
b1299 puuR +0.5 2.2 DNA-binding transcriptional repressor (NCBI) compare
b2187 yejL +0.5 1.6 hypothetical protein (NCBI) compare
b2141 yohJ +0.5 1.7 hypothetical protein (NCBI) compare
b3100 yqjK +0.5 1.9 hypothetical protein (NCBI) compare
b1712 ihfA +0.5 0.7 integration host factor subunit alpha (NCBI) compare
b3149 yraO +0.5 2.9 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1591 b1591 +0.5 1.2 putative oxidoreductase component (VIMSS) compare
b3101 yqjF +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b3781 trxA +0.5 1.6 thioredoxin 1 (VIMSS) compare
b1233 ychJ +0.5 1.8 hypothetical protein (NCBI) compare
b1306 pspC +0.5 1.6 DNA-binding transcriptional activator (NCBI) compare
b3688 yidQ +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b1795 yeaQ +0.5 2.2 conserved inner membrane protein (NCBI) compare
b0927 ycbL +0.5 1.6 predicted metal-binding enzyme (NCBI) compare
b1180 ycgM +0.5 1.4 predicted isomerase/hydrolase (NCBI) compare
b0579 ybdF +0.5 1.7 hypothetical protein (NCBI) compare
b2262 menB +0.5 1.5 naphthoate synthase (NCBI) compare
b0885 aat +0.5 2.2 leucyl/phenylalanyl-tRNA--protein transferase (NCBI) compare
b0348 mhpB +0.5 1.5 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (NCBI) compare
b1388 paaA +0.5 2.0 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b1917 yecC +0.5 1.9 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4394 yjjX +0.5 1.1 orf, hypothetical protein (VIMSS) compare
b1802 yeaW +0.5 2.0 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b2044 wcaL +0.5 2.0 predicted glycosyl transferase (NCBI) compare
b4325 yjiC +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b2061 wzb +0.5 1.1 protein-tyrosine phosphatase (NCBI) compare
b0331 prpB +0.5 2.1 2-methylisocitrate lyase (NCBI) compare
b1161 ycgX +0.4 0.5 hypothetical protein (NCBI) compare
b0195 yaeB +0.4 2.4 hypothetical protein (NCBI) compare
b2455 eutE +0.4 1.5 predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI) compare
b3918 cdh +0.4 3.0 CDP-diacylglycerol pyrophosphatase (NCBI) compare
b0209 yafD +0.4 2.2 hypothetical protein (NCBI) compare
b4596 yciZ +0.4 0.8 hypothetical protein (RefSeq) compare
b1526 yneJ +0.4 1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1728 ydjM +0.4 1.3 predicted inner membrane protein regulated by LexA (RefSeq) compare
b4371 rsmC +0.4 2.8 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b1262 trpC +0.4 2.4 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b4174 hflK +0.4 2.8 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3257 yhdT +0.4 1.2 conserved inner membrane protein (NCBI) compare
b0191 yaeJ +0.4 1.8 hypothetical protein (NCBI) compare
b2614 grpE +0.4 0.9 heat shock protein (NCBI) compare
b1656 sodB +0.4 1.0 superoxide dismutase, Fe (NCBI) compare
b4513 kdpF +0.4 0.5 potassium ion accessory transporter subunit (NCBI) compare
b2698 recX +0.4 0.9 RecA regulator RecX (NCBI) compare
b1647 ydhF +0.4 1.9 predicted oxidoreductase (NCBI) compare
b2708 gutQ +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1988 nac +0.4 1.9 DNA-binding transcriptional dual regulator of nitrogen assimilation (NCBI) compare
b1038 csgF +0.4 1.2 predicted transport protein (NCBI) compare
b0842 cmr +0.4 2.8 multidrug efflux system protein (NCBI) compare
b2094 gatA +0.4 2.1 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b0784 moaD +0.4 1.4 molybdopterin synthase, small subunit (NCBI) compare
b0756 galM +0.4 2.1 galactose-1-epimerase (mutarotase) (NCBI) compare
b0219 yafV +0.4 1.3 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b1846 yebE +0.4 1.7 hypothetical protein (NCBI) compare
b1181 ycgN +0.4 2.7 conserved protein (RefSeq) compare
b2195 ccmG +0.4 1.2 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b2674 nrdI +0.4 1.7 hypothetical protein (NCBI) compare
b0211 mltD +0.4 2.4 predicted membrane-bound lytic murein transglycosylase D (NCBI) compare
b1305 pspB +0.4 0.8 phage shock protein B (NCBI) compare
b4418 sraB +0.4 1.0 unknown RNA (NCBI) compare
b3926 glpK +0.4 3.2 glycerol kinase (NCBI) compare
b4464 ygfQ +0.4 2.3 predicted transporter (NCBI) compare
b0942 ycbU +0.4 1.8 predicted fimbrial-like adhesin protein (NCBI) compare
b3884 yihW +0.4 2.3 putative DEOR-type transcriptional regulator (VIMSS) compare
b4566 yjhX +0.4 2.4 hypothetical protein (NCBI) compare
b2026 hisI +0.4 1.7 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI) compare
b3033 yqiB +0.4 0.8 predicted dehydrogenase (NCBI) compare
b2240 glpT +0.4 3.0 sn-glycerol-3-phosphate transporter (NCBI) compare
b4568 ytjA +0.4 1.0 predicted protein (RefSeq) compare
b4521 ycgI +0.4 1.1 hypothetical protein (NCBI) compare
b1064 grxB +0.4 2.0 glutaredoxin 2 (Grx2) (NCBI) compare
b1629 rsxC +0.4 1.9 electron transport complex protein RnfC (NCBI) compare
b1303 pspF +0.4 1.7 psp operon transcriptional activator (VIMSS) compare
b1887 cheW +0.4 2.5 purine-binding chemotaxis protein (NCBI) compare
b2003 yeeT +0.4 1.7 CP4-44 prophage; predicted protein (NCBI) compare
b1295 ymjA +0.4 0.6 hypothetical protein (NCBI) compare
b3014 yqhH +0.4 2.1 predicted outer membrane lipoprotein (NCBI) compare
b2823 ppdC +0.4 1.5 hypothetical protein (NCBI) compare
b1251 yciI +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b2946 yggJ +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b0228 yafM +0.4 1.2 hypothetical protein (NCBI) compare
b0843 ybjH +0.4 0.8 hypothetical protein (NCBI) compare
b0775 bioB +0.4 1.4 biotin synthase (NCBI) compare
b1485 ddpC +0.4 1.2 D-ala-D-ala transporter subunit (NCBI) compare
b1349 recT +0.4 1.9 Rac prophage; recombination and repair protein (NCBI) compare
b4595 yciY +0.4 0.5 hypothetical protein (RefSeq) compare
b4416 rybA +0.4 0.7 unknown RNA (NCBI) compare
b4398 creB +0.4 2.6 DNA-binding response regulator in two-component regulatory system with CreC (NCBI) compare
b0825 mipB +0.4 1.3 putative transaldolase (VIMSS) compare
b0991 ymcE +0.4 1.0 cold shock gene (NCBI) compare
b1184 umuC +0.4 2.1 DNA polymerase V subunit UmuC (NCBI) compare
b4651 yibW +0.4 0.2 no description compare
b3527 yhjJ +0.4 3.4 predicted zinc-dependent peptidase (NCBI) compare
b3623 rfaK +0.4 0.5 lipopolysaccharide core biosynthesis (NCBI) compare
b2208 napF +0.4 1.8 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare


Specific Phenotypes

For 6 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms