Experiment set10IT004 for Escherichia coli BW25113

LB Aerobic with Phenazine ethosulfate 0.0000625 mM

Group: stress
Media: LB + Phenazine ethosulfate (6.25E-02 mM)
Culturing: Keio_ML9a, 96 deep-well microplate; Multitron, Aerobic, at 37 (C), shaken=750 rpm
By: Hans_Hualan on 7/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 24 genes in this experiment

For stress Phenazine ethosulfate in Escherichia coli BW25113

For stress Phenazine ethosulfate across organisms

SEED Subsystems

Subsystem	#Specific
Transport of Iron	3
Iron acquisition in Vibrio	2
Oxidative stress	2
Biogenesis of cytochrome c oxidases	1
Campylobacter Iron Metabolism	1
Capsular heptose biosynthesis	1
D-allose utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Glycine and Serine Utilization	1
LOS core oligosaccharide biosynthesis	1
Lipid A modifications	1
Nitric oxide synthase	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Pyruvate Alanine Serine Interconversions	1
Staphylococcal phi-Mu50B-like prophages	1
Thioredoxin-disulfide reductase	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Galactose metabolism
• Photosynthesis
• Purine metabolism
• Aminophosphonate metabolism
• Selenoamino acid metabolism
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Inositol phosphate metabolism
• Glycerophospholipid metabolism
• Sphingolipid metabolism
• Folate biosynthesis
• Biosynthesis of ansamycins

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
cis-cyclopropane fatty acid (CFA) biosynthesis	1	1	1
superoxide radicals degradation	2	2	1
sterculate biosynthesis	2	1	1
cardiolipin biosynthesis I	3	3	1
cardiolipin biosynthesis II	3	3	1
reactive oxygen species degradation	4	4	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane)	4	1	1
GDP-D-glycero-α-D-manno-heptose biosynthesis	4	1	1
photosynthesis light reactions	4	1	1
ADP-L-glycero-β-D-manno-heptose biosynthesis	5	5	1
superpathway of photosynthetic hydrogen production	6	3	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	2
ethene biosynthesis III (microbes)	7	6	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	1
oxygenic photosynthesis	17	11	1
ethene biosynthesis V (engineered)	25	18	1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered)	26	19	1
1-butanol autotrophic biosynthesis (engineered)	27	19	1
mycolate biosynthesis	205	25	3
superpathway of mycolate biosynthesis	239	26	3