Protein Info for UW163_RS10075 in Ralstonia solanacearum UW163

Annotation: lipid A export permease/ATP-binding protein MsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 transmembrane" amino acids 31 to 51 (21 residues), see Phobius details amino acids 75 to 100 (26 residues), see Phobius details amino acids 143 to 168 (26 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 260 to 281 (22 residues), see Phobius details amino acids 287 to 305 (19 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 19 to 590 (572 residues), 892.9 bits, see alignment E=4.2e-273 PF00664: ABC_membrane" amino acids 35 to 305 (271 residues), 151.4 bits, see alignment E=4.3e-48 PF00005: ABC_tran" amino acids 366 to 518 (153 residues), 119.2 bits, see alignment E=2.3e-38

Best Hits

Swiss-Prot: 98% identical to MSBA_RALSO: Lipid A export ATP-binding/permease protein MsbA (msbA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 100% identity to rsc:RCFBP_11196)

MetaCyc: 41% identical to ATP-binding lipopolysaccharide transport protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-236 [EC: 7.5.2.6]; 7.5.2.6 [EC: 7.5.2.6]

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA" in subsystem ZZ gjo need homes

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 7.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (592 amino acids)

>UW163_RS10075 lipid A export permease/ATP-binding protein MsbA (Ralstonia solanacearum UW163)
MAVTSSSSSQPPATSQPGHFKRLWAYLRPELASFILAMVAMGVVAATEGIIPKVVKDLLD
QGFGGEYAGKLWRVPAMLVGIAVVRGVAQFGATYFLSLVSNKVLLNLRMKMFERLLQAPA
AFYQRNTGASLINAVIFEVNQVLQVLTGVFITLVRDSMTVVALLIFLFYTNWRLTLVVAV
ILPVIGFLMSRINRRLRSLNREHQNLTNEAAYVVEEAAGGYKVVKLHGGEAYESRRFNAM
TNRLRGYAMRMAVAGGLNQPVTQFLAALALSVILAIAMVQAQANQTTVGGFTGFVMAMLL
LISPLKHLTDVNQPLQRGLTAAEFIFGLIDTPIEPQEGGKHIDRARGDLRFEHVTFRYGP
DGRAALDDIDLHVKAGEIVALVGPSGSGKTTLVNLLPRFFEPTSGRIVLDGDALADLSLQ
DLRRQIAFVSQDVVLFNDTIAANVAYGARDASEIDMARVRRALEAAYLTDVVDNLPEGVD
TNIGDNGSKLSGGQRQRLAIARAIYKDAPILILDEATSALDSESERQVQAALEALMQGRT
TLVIAHRLSTIENADRIVVLEHGKIVEAGTHRELIDRDGLYAGLHRIQFATQ