Protein Info for UW163_RS06400 in Ralstonia solanacearum UW163

Annotation: DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 23 to 630 (608 residues), 782.6 bits, see alignment E=2.3e-239 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 25 to 484 (460 residues), 495.9 bits, see alignment E=1.2e-152 PF00270: DEAD" amino acids 39 to 198 (160 residues), 89.5 bits, see alignment E=5.4e-29 PF00271: Helicase_C" amino acids 256 to 349 (94 residues), 64.2 bits, see alignment E=3.3e-21 PF16124: RecQ_Zn_bind" amino acids 361 to 422 (62 residues), 70.2 bits, see alignment E=5.1e-23 PF09382: RQC" amino acids 424 to 538 (115 residues), 125.4 bits, see alignment E=2.6e-40 PF00570: HRDC" amino acids 565 to 630 (66 residues), 75.2 bits, see alignment E=8.2e-25

Best Hits

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 98% identity to rsc:RCFBP_10424)

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (637 amino acids)

>UW163_RS06400 DNA helicase RecQ (Ralstonia solanacearum UW163)
MFDSPAIDHALDALPEDTAAATRAVLHDVFGYSAFRGPQADIVTHVADGGDCLVLMPTGG
GKSLCYQIPALVRHRRGQGAGIVVSPLIALMQDQVAALEEAGVRAAYLNSALTGTEAAQV
ERDLAAGRLDLVYVAPERLMTPRFLELLERSRIGLFAIDEAHCVSQWGHDFRPEYIQLSV
LHERFPHVPRIALTATADAVTRDEIVERLALGGERVFLSSFDRPNIRYTIVEKDSARQQL
LRFIRAEHLGEDGCDAGIVYCLSRKKVEETAQWLAEQGIRALPYHAGMDVETRARHQAIF
RKEEGVVMVATIAFGMGIDKPDVRFVAHLDLPKSLEGYYQETGRAGRDGLPANAWMAYGL
ADVVQQRRMIDESDADDVHKRVSTAKLDALLGLCESAGCRRVALLAYFGESSQPCGNCDT
CLSPPQTWDATREAQMALSCVYRVQQASRVSFGAGQLIDILRGNATERVKQWHHETLSTF
GVGDTLSEAAWRGVFRQLVAQGLLAVDHGGHGALVLTDAARPVLKGEHSVTLRRQAEKVR
AAPASGAKAERRADPAADLSVEAQMRWQALRVWRAQTAREHSVPAYVIFHDSTLAHIAES
DPASREALAELPGIGVAKLDRYGQALLDVLAKCRGES