Protein Info for UW163_RS04880 in Ralstonia solanacearum UW163

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 768 PF17764: PriA_3primeBD" amino acids 24 to 117 (94 residues), 82.7 bits, see alignment E=4.1e-27 PF04851: ResIII" amino acids 222 to 365 (144 residues), 39.4 bits, see alignment E=1.8e-13 PF00270: DEAD" amino acids 245 to 388 (144 residues), 58.3 bits, see alignment E=2.6e-19 TIGR00595: primosomal protein N'" amino acids 246 to 766 (521 residues), 553.8 bits, see alignment E=1.8e-170 PF18319: PriA_CRR" amino acids 479 to 505 (27 residues), 39.9 bits, see alignment (E = 9.4e-14) PF00271: Helicase_C" amino acids 538 to 623 (86 residues), 27.9 bits, see alignment E=7.5e-10 PF18074: PriA_C" amino acids 666 to 765 (100 residues), 57.6 bits, see alignment E=5.4e-19

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 97% identity to rsc:RCFBP_10166)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (768 amino acids)

>UW163_RS04880 primosomal protein N' (Ralstonia solanacearum UW163)
MTATRVDADPPTAAQAARPAAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGNRRVVA
LVVATADTTEVPADRLRDIERVLDWVPPLDAEWRALAEFAASYYHRALGEVTLPALPPLW
RTPGSWDNLARQATETVYLMHEPARAALLDSIPRRAAGAMRLAEALTGDGELSASAAVAL
HGQAMAKLRDWATVGWVRAELRPKSLLPVPHDLPPPSAAPPLNAEQAAAVAGIAQAGAGG
GFAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVP
LAALHSGLAEQPRALNWLAAHRGEARIVLGTRLAMLASLPNLALILVDEEHDTSYKQQEG
LRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGATTRLVLSQRAQADAVLPRVLL
IDLTLERRAGREIRDGLSKPLLDAVADCLARGEQSLLFLNRRGYAPVLACDACGWLSGCP
RCSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALFPQAR
VARIDADSTRRKGSAEALFDTVHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDSSLFSH
DFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPALLALVRHDYAGFARQLLRERK
QACLPPFAYQALLTAEHRELARALEFLGAAREAGQALAEAQGLPVQLHDPVPMTMVRLAN
RERAQLLLESGSRAALQRLLAAWTDGFGEIGKTVKGVRWQLEIDPLRI