Protein Info for RPSI07_RS21420 in Ralstonia solanacearum PSI07

Annotation: glycine--tRNA ligase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 TIGR00388: glycine--tRNA ligase, alpha subunit" amino acids 3 to 313 (311 residues), 492.3 bits, see alignment E=2.3e-152 PF02091: tRNA-synt_2e" amino acids 4 to 306 (303 residues), 471.9 bits, see alignment E=3.3e-146

Best Hits

Swiss-Prot: 99% identical to SYGA_RALSO: Glycine--tRNA ligase alpha subunit (glyQ) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01878, glycyl-tRNA synthetase alpha chain [EC: 6.1.1.14] (inferred from 100% identity to rsl:RPSI07_2838)

Predicted SEED Role

"Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (325 amino acids)

>RPSI07_RS21420 glycine--tRNA ligase subunit alpha (Ralstonia solanacearum PSI07)
MLTFQQLILKLQSYWDAQGCALLQPIDLEVGAGTSHVHTFLRAIGPEPWRAAYVQPSRRP
KDGRYGENPNRLQHYYQYQVVLKPAPENILDLYLGSLQALGLDLKQNDVRFVEDDWENPT
LGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCKPITGEITYGIERLAMYLQQVENIYDLVW
TEWEEPGPNGPVKRRLTYGDVYHQNEVEQSTYNFEHADTTVLFRRFAEHEAEAKRLMGVR
EEGAADDGAPVPQLALPAYEQVLKAGHTFNLLDARGAISVTERAAYIGRIRNLSRLVAQA
YYDSRERLGFPMCGTAAAPAATEAA