Protein Info for RPSI07_RS17675 in Ralstonia solanacearum PSI07

Annotation: translation initiation factor IF-2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 964 PF04760: IF2_N" amino acids 1 to 52 (52 residues), 35.6 bits, see alignment 2.3e-12 amino acids 386 to 436 (51 residues), 59 bits, see alignment 1.1e-19 PF08364: IF2_assoc" amino acids 62 to 100 (39 residues), 52.2 bits, see alignment (E = 1.9e-17) TIGR00487: translation initiation factor IF-2" amino acids 381 to 964 (584 residues), 879.3 bits, see alignment E=1.5e-268 TIGR00231: small GTP-binding protein domain" amino acids 466 to 623 (158 residues), 111.9 bits, see alignment E=2.6e-36 PF00009: GTP_EFTU" amino acids 469 to 624 (156 residues), 133.5 bits, see alignment E=2.5e-42 PF01926: MMR_HSR1" amino acids 469 to 574 (106 residues), 39.6 bits, see alignment E=1.8e-13 PF00071: Ras" amino acids 470 to 625 (156 residues), 23.9 bits, see alignment E=9.8e-09 PF11987: IF-2" amino acids 740 to 854 (115 residues), 148.5 bits, see alignment E=2.7e-47 PF03144: GTP_EFTU_D2" amino acids 885 to 952 (68 residues), 32.5 bits, see alignment 3.2e-11

Best Hits

Swiss-Prot: 76% identical to IF2_BURM1: Translation initiation factor IF-2 (infB) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 76% identity to bmj:BMULJ_01485)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (964 amino acids)

>RPSI07_RS17675 translation initiation factor IF-2 (Ralstonia solanacearum PSI07)
MASTTVAQLAGELNRSAAALLEQLQAAGVQKATPEDVITESDKTRLLDYLKRAHGSAEDG
ARKKITLTKRETSEIRQADATGKTRTVQVEVRKKRVLMKRDEAGAAQAEAEAPAQVAQVV
DAEEVARREEEQRRQSELLARQEAELKARQEAMEREEAERRARQEAAEAEQRRQAELAAK
KAEEEAAAARAAAEASDEAPRRKAEEDAARLASEREAAQKASEEARAAADKIKAEEDAAR
KRREAAEAEARAIREMMNAPARVLKTPAERKAEEKKAEQSGTLHKPVKAAGEARPAPAVK
KVAAPATTPAAAPAGDKKGGRGGKAGGWQDDSRGSKRGGLKTRGDTGGGVDGWRAGSKGG
RNRHGDDNRNAFQAPTEPVVREVHVPETISVADLAHKMAVKAAEVIKQMMKLGQMVTINQ
VLDQETAMIVVEEMGHQAIAAKLDDPEALLIEGGDEQADVEAETRPPVVTVMGHVDHGKT
SLLDYIRRAKVAAGEAGGITQHIGAYHVETPRGVITFLDTPGHEAFTAMRARGAKATDIV
ILVVAADDGVMPQTKEAIAHAKAAGVPIVVAINKIDKPEANPDRVKQELVSESVIPEEYG
GDSPFVPVSAKTGQGIEELLENVLLQAEVLELKAPISAPAKGLVVEAQLDKGKGPIATVL
VQSGTLKRGDVVLAGTAYGRVRAMLDENGKPAKEAGPSIPVEIQGLSEVPGAGEEVIVLP
DERKAREIALFRQGKFRDVKLARQQAAKLETMLEQMSEGEVKALPLIIKADVQGSQEALV
HALNKLSTGEVRVQVVHGAVGGISESDVNLATASKAVIIGFNTRADAGARKLAEHQGIDI
RYYNIIYDAVDEVKAAMSGMLSPEKKEETTGLVEVRQVFHVPKVGAVAGCMVLDGIVKRN
SLVRVLRENVVIFSGELESLKRFKDDVKEVKQGFECGLSIKNFNDVKEGDQLEIYEITEV
ARTL