Protein Info for RPSI07_RS17115 in Ralstonia solanacearum PSI07

Annotation: 16S rRNA uridine-516 pseudouridylate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 641 PF01479: S4" amino acids 389 to 435 (47 residues), 33.7 bits, see alignment 2.4e-12 PF00849: PseudoU_synth_2" amino acids 451 to 606 (156 residues), 55.3 bits, see alignment E=9.1e-19 TIGR00093: pseudouridine synthase" amino acids 489 to 635 (147 residues), 114.4 bits, see alignment E=2.2e-37

Best Hits

KEGG orthology group: K06182, ribosomal large subunit pseudouridine synthase F [EC: 5.4.99.12] (inferred from 90% identity to rsl:RPSI07_1961)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (641 amino acids)

>RPSI07_RS17115 16S rRNA uridine-516 pseudouridylate synthase (Ralstonia solanacearum PSI07)
MTDSDDIPGDDTKRLANDPTQRATRPGKRATLGVRRDDTGNPVRTTGKPLRASDLNRDRQ
PLRPSQRRPKPQEGAGNKPAQPRRDDDGRPPREAKPRRMQGDRPPRRFDDERPPRKFGDD
QRPPRRFGDERPPRRFEDRPPRRFDDERPPRKFGDDQRPPRRFEDERPPRRFEDRPPRRF
DDERPPRKFGDDQRPPRRFGDERPPRRFEDRPPRRFDDERPPRKFGDDQRPPRRFGDERP
PRRFEDRPPRRFDDERPPRKFGDDQRPPRRFEDERPPRRFEDRPPRRFDDERPPRKFGDD
QRPPRRFGDERPPRRFEDRPPRRFDDERPPRKFGDDQRPPRRFEDERPPRRFEDRPARAP
RPEREERAPRRAEEHTPHPAPQAESSGLVRLSKRMSELGLCSRREADEWIPRGWVLVDGE
PVTELGSRVRPDAEIEILPAARSEQGERVTVLLHKPVGYVSGQAEDGYEPAAALFTAENQ
WEEDPTHKRFAAWQHKGLAPAGRLDIDSTGLLVLTQDGRVARHLIGEDSTVEKEYLVRVV
WDAPQGPVEHDVSKVFPEELLEKLRHGLSLDGEALKPAKVSWQNEEQLRFVLREGKKRQI
RRMCEQVGLEVVGLKRIRMGRVVLGDLPVGKWRFLGRFEKF