Protein Info for RPSI07_RS16400 in Ralstonia solanacearum PSI07

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF00561: Abhydrolase_1" amino acids 37 to 298 (262 residues), 113.9 bits, see alignment E=1.6e-36 PF12146: Hydrolase_4" amino acids 38 to 145 (108 residues), 30.2 bits, see alignment E=4.4e-11 PF12697: Abhydrolase_6" amino acids 40 to 302 (263 residues), 72.5 bits, see alignment E=1.3e-23

Best Hits

KEGG orthology group: None (inferred from 100% identity to rsl:RPSI07_1813)

Predicted SEED Role

"Epoxide hydrolase (EC 3.3.2.9)" (EC 3.3.2.9)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (311 amino acids)

>RPSI07_RS16400 alpha/beta hydrolase (Ralstonia solanacearum PSI07)
MTAATATAAATITHPQFADLPNGTRLHYASAGRRGAPLMLFVHGFPEFWYAWEAQLAAFG
DTHFAVAPDMRGYNLSSKPTAVDAYRPKLLVEDLEQFITALGYERAIVVAHDWGGAISWN
LAIQHPERVERLVIVNAPHPWVFANALLTDPAQQAASAYMNWLRQPGVEDILAADEFDKL
EGFFHGMGQPVAEWFTTDVRARYHAAWSRPGEGGSHGLTGGVNYYRASPLHPPAEGLAPL
RIDQMPPEAFVVKMPTLVVWGEKDMALPATLLNGLGRFIPDLRIERIPDGTHWVLHEQPA
RITALIRRFVA