Protein Info for RPSI07_RS13870 in Ralstonia solanacearum PSI07

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 810 PF13328: HD_4" amino acids 115 to 264 (150 residues), 175.6 bits, see alignment E=1.9e-55 TIGR00691: RelA/SpoT family protein" amino acids 115 to 804 (690 residues), 732.7 bits, see alignment E=2.1e-224 PF01966: HD" amino acids 134 to 233 (100 residues), 43.1 bits, see alignment E=1.5e-14 PF04607: RelA_SpoT" amino acids 324 to 434 (111 residues), 138.3 bits, see alignment E=3.9e-44 PF02824: TGS" amino acids 477 to 536 (60 residues), 71.6 bits, see alignment 1.3e-23 PF19296: RelA_AH_RIS" amino acids 549 to 698 (150 residues), 34.1 bits, see alignment E=9e-12 PF13291: ACT_4" amino acids 726 to 805 (80 residues), 60.4 bits, see alignment E=6.7e-20

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to rsl:RPSI07_1272)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (810 amino acids)

>RPSI07_RS13870 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Ralstonia solanacearum PSI07)
MPTSASGSASPRTGAPNLPDPHGADLVGERAALPPRLPKAVSHGTDASVPAVARAESPVG
ETLFIDAVLEQTYRHLFGPTSQPAAPPRQQVVSIAGLTEKLSYLKPADMKLVKEAFHFSD
EAHLGQYRQSGEPYITHPVAVAEICAGWKLDVQSIMAALLHDVIEDQGVTKSELAEKFGP
KVAELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVP
PEKRRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSSFRYATLEKAVKAARGNRRE
VVSRILEAAQRALGDAGIPAELTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVETQM
QCYMAVGALHSLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTREMNQIA
EAGVAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQSGDSQEFLEHVKIDLFPDAVYVF
TPKGEIRALPRGATALDFAYAVHSDLGNQCVAVKINNALLPLRTELKNGDIVEVVTAPYS
KPNPAWLTFVRTGKARAAIRHFLKTAKLDEAIQLGERLLEQALRQLGIDMKAVPAQVWER
IVQWTGNKAREDVFADLALGRRVAAVVARRIEIVLQDGSYEGGDALIATVHAFAGEEAPA
VTVSGDEGMAMVFSPCCRPIPGDPIVGYIGKGEGLQIHVEECRVAKRLHGKDPEHWIDVM
WAEHTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQGGGNQEATYMTFLIQ
VHDRVHLADVMRALRRNPDVIRIARDRGGE