Protein Info for RPSI07_RS13550 in Ralstonia solanacearum PSI07

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 603 TIGR01391: DNA primase" amino acids 1 to 422 (422 residues), 446.2 bits, see alignment E=5.6e-138 PF01807: zf-CHC2" amino acids 3 to 97 (95 residues), 133.9 bits, see alignment E=6.1e-43 PF08275: DNAG_N" amino acids 127 to 257 (131 residues), 123.1 bits, see alignment E=3.2e-39 PF13662: Toprim_4" amino acids 266 to 333 (68 residues), 62.3 bits, see alignment E=1.6e-20 PF13362: Toprim_3" amino acids 267 to 361 (95 residues), 27.8 bits, see alignment E=1.1e-09 PF01751: Toprim" amino acids 267 to 343 (77 residues), 51.6 bits, see alignment E=3.4e-17 PF13155: Toprim_2" amino acids 269 to 356 (88 residues), 62.1 bits, see alignment E=2.1e-20 PF10410: DnaB_bind" amino acids 375 to 428 (54 residues), 44.3 bits, see alignment 6.8e-15 PF08278: DnaG_DnaB_bind" amino acids 465 to 592 (128 residues), 68.3 bits, see alignment E=3.4e-22

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 100% identity to rsl:RPSI07_1206)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (603 amino acids)

>RPSI07_RS13550 DNA primase (Ralstonia solanacearum PSI07)
MIPQPFIDDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFSVSPTKQFYHCFG
CGAHGSAIGFLMEFSGLSYVEAIRELAQSVGVAVPEERGGLPPAARAERQAKTVALGDVM
ARASDHYRKQLRSAPNAVQYLKGRGLTGEIAGHFGLGYAPDDWQSLEAVFGAYRDDAIAV
PLIEAGLVIESEKTDADGRHRRYDRFRDRIMFPIRNTKGVVIGFGGRVLGQGEPKYLNSP
ETPLFSKGTELYGLFEARNAIREFGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHV
QKLLRQVDTVIFSFDGDAAGRRAARRALEACLPHVTDNKTVKFLFLPPEHDPDTFIREEG
TEAFAAEVRNAMPLSRFLLQAVTEDLDLRQPEGRARAQYEAKPLLSAMPPGGLRLQIVRG
LAEMTGTTPADIEALCGLRSDPAQAGRMTQKRPRVARTAPTALEQKVLRLLMRYPALAAR
LDVDTRTQLTAPELPQGEMLSGLLAECDVAGPEMHFGAFVERLGQTPYAEAFAGLRMAVL
NDDIELEPATQEFDAAVQKMLAEPLRRELDMLQRKMEGGHASDADKERLRWLVSEISRRR
RLV