Protein Info for RPSI07_RS12605 in Ralstonia solanacearum PSI07

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 960 TIGR00392: isoleucine--tRNA ligase" amino acids 24 to 874 (851 residues), 850.6 bits, see alignment E=7.2e-260 PF00133: tRNA-synt_1" amino acids 36 to 672 (637 residues), 427.6 bits, see alignment E=1.2e-131 PF09334: tRNA-synt_1g" amino acids 67 to 207 (141 residues), 41.1 bits, see alignment E=2.6e-14 PF08264: Anticodon_1" amino acids 717 to 873 (157 residues), 86.6 bits, see alignment E=4.1e-28 PF06827: zf-FPG_IleRS" amino acids 922 to 948 (27 residues), 29.1 bits, see alignment (E = 1.8e-10)

Best Hits

Swiss-Prot: 82% identical to SYI_CUPMC: Isoleucine--tRNA ligase (ileS) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 72% identity to bcm:Bcenmc03_2537)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (960 amino acids)

>RPSI07_RS12605 isoleucine--tRNA ligase (Ralstonia solanacearum PSI07)
MSDSKKTPAPEKNKYPVNLLDTPFPMRGDLPKREPQWVKQWQEKQLYKKIRAARKGAKKF
ILHDGPPYANGDLHIGHAVNKILKDMVIKSRGLAGLDAVYVPGWDCHGMPIEIQIEKQFG
KGLPVKEVQEKARAYAATQIARQKVDFERLGVLGDWADPYLTMNFRNEADEVRALGKILE
KGYVFRGLKPVNWCFDCGSALAEAEVEYADRTDLSIDVGFPFADIDALASAFHVGTDVLK
AKPGWIVIWTTTPWTIPSNQALNLHPEIEYALVDTPRGLVVVARERVEACLQSWKLEGTV
LATCEGAALSLVRFHHPLAKTDAGYDRLSPVYLGDYVTTDSGTGIVHSAPAYGVEDFESC
KAHDMPDADIINPVMGNGVYASTLPLFGGLSIWDACPKIVEVLKESGNLFDVHQYTHSYM
HCWRHKTPIIYRATSQWFAGMDVQPNDGNATLRETALTGIDATAFYPAWGKQRLHNMIAN
RPDWTLSRQRQWGVPMAFFVHRETGALHPRTPELLEHVAQRIEKSGVEAWQTLDPRELLG
DEADMYEKNRDTLDVWFDSGTTHWHVIRGSHAADLYDAAAELPEGRLADLYLEGSDQHRG
WFHSSLLTASMLYGKPPYKALLTHGFTVDGEGRKMSKSIGNTIAPQEIAGKMGAEIIRLW
VASTDYSGELAISDEILKRVVEGYRRIRNTLRFLLANLTDYDHARHALPPEQWLEIDRYA
VALTDALQKEILSHYDVYEFHPVVSKLQTFCSEDLGGFYLDVLKDRLYTTAPDSVARRSA
QNALYHITQAMLHWMAPFLSFTAEEAWQVFAHGTQHTDTIFTSTYYDAPVPQGASALLEK
WATIRNVRGEVTKQLEALRIDGKIGSSLQAEVTVSAGDGVYNALAGLGDDLRFVLITSAA
KLERAPEGGDLLISVVPSGHAKCERCWHYRADVGHDLAHPTLCKRCTDNLFGAGEQRSAA