Protein Info for RPSI07_RS11015 in Ralstonia solanacearum PSI07

Annotation: preprotein translocase subunit SecA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 934 PF07517: SecA_DEAD" amino acids 6 to 405 (400 residues), 439.8 bits, see alignment E=1.1e-135 TIGR00963: preprotein translocase, SecA subunit" amino acids 28 to 828 (801 residues), 1118.6 bits, see alignment E=0 PF01043: SecA_PP_bind" amino acids 232 to 361 (130 residues), 132.7 bits, see alignment E=2.1e-42 PF21090: P-loop_SecA" amino acids 422 to 625 (204 residues), 313.2 bits, see alignment E=1.9e-97 PF07516: SecA_SW" amino acids 627 to 839 (213 residues), 227.3 bits, see alignment E=4.9e-71 PF02810: SEC-C" amino acids 914 to 932 (19 residues), 40 bits, see alignment (E = 7.2e-14)

Best Hits

Swiss-Prot: 74% identical to SECA_BURCJ: Protein translocase subunit SecA (secA) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 74% identity to bam:Bamb_0472)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (934 amino acids)

>RPSI07_RS11015 preprotein translocase subunit SecA (Ralstonia solanacearum PSI07)
MITGLLKKIFGSRNERLIKQYRRKVAQINALEPTLEVLSDAELQAKTEAFRQRFAKGETL
DALLPEAFAVCREASKRVMKMRHFDVQLIGGMVLHDGKIAEMRTGEGKTLTATLAVYLNA
IAGQGVHVVTVNDYLAQRDAEWMGKLYNWLGLSVGVNLTTMAHDEKQAAYAADITYGTNN
EFGFDYLRDNMVYDASQRVQRPLNYAIVDEVDSILIDEARTPLIISGQAEDHTDLYRRMN
SIPPLLTRQIGEEKADGTGVEKPGDYYVDEKSHQVYLTEAGHEKAEQILLQAGLLAEGES
LYAPQNITLMHHLYASLRAHSLFHRDQHYVVQSDEVIIVDEFTGRLMQGRRWSDGLHQAV
EAKEGVQIQQENQTLATITFQNYFRMYAKLSGMTGTADTEAYEFQEIYALETVVIPTNRL
AQRKDLQDQIYKTAKERYDAVIRDIRDCYERSQPVLVGTTSIENSELLSHLLNQAQLPHQ
VLNAKQHAREAEIIAQAGRPKMITIATNMAGRGTDIVLGGNVEKQAGFVMVDESLSDEEK
AKRVTQLQDEWQSLHEQVKAAGGLHIVGTERHESRRIDNQLRGRAGRQGDPGSSRFYLSL
DDQLLRIFAGDRVRAIMDRLKMPEGEPIEAGIVTRSIESAQRKVEGRNFDIRKQLLQYDD
VANDQRREIYKLRNEILESVDAGDLVKNLRESVFVELFRTYVPAETMEEQWDLAGLEKTL
RDDWGVEVPLLKTVEQAQSIEDEDLLTQVREAVEAVYAGKVAQVGREAFAGFERSVMLQS
LDTHWREHLAALDHLRQGIHLRGYAQKDPKQEYKRESFELFGRLLDTIRNEVTRIIFTVR
IQSQEALEEASEQIEEDLSHLTNVQYKHDEFSELAEVAAGDAEIHGETPAMPAHRSAAAS
AAAALAGQVPKVGRNDPCPCGSGKKYKQCHGKLA