Protein Info for QEN71_RS41370 in Paraburkholderia sabiae LMG 24235

Annotation: diaminopropionate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01747: diaminopropionate ammonia-lyase family
  • amino acids 42 to 389 (348 residues), 283.4 bits, E=1.2e-88
• PF00291: PALP
  • amino acids 43 to 376 (334 residues), 208.8 bits, E=5.9e-66

Best Hits

KEGG orthology group: K01751, diaminopropionate ammonia-lyase [EC: 4.3.1.15] (inferred from 85% identity to bug:BC1001_2056)

Predicted SEED Role

"Threonine dehydratase (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Threonine degradation (EC 4.3.1.19)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-methionine degradation II (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• L-threonine degradation V (1/2 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.15, 4.3.1.19

Use Curated BLAST to search for 4.3.1.15 or 4.3.1.19

Protein Sequence (409 amino acids)

>QEN71_RS41370 diaminopropionate ammonia-lyase (Paraburkholderia sabiae LMG 24235)
MLVANPRATRSPYPDALKSILNIEKAEESRSWLFCWDKICADATPLRELPGLSAKLHIGS
ISVKDESARSVLGSFKALGAPIALVRLIQRLWPDHVLDARDLFEGRYRELLTHFTVVSAT
DGNHGKGLAAAAQTLGCHCVIVLHANVSVEREQAIAAYGAEIVRIAGNYDESVEEAARLA
AVNGWHVVSDTSYDGYEDIPRDVMQGYGTIAAEIVAQSDSHPDRPAYTHVFLQGGVGGLA
AGLASYLWEFHGEHRPRFIVVEPQQADCLYQSALAGRAARATGSVDSVMAGLACGETSPL
AWRFLQPSVDDFMTISDDDAVSAMRTLAAGADNDVPIVAGESGVAGLAGLIVLLQDQQLA
RKVGLNAASRVLIVNTEGATAPGTYQALVGEAASSVLERQSAWRVNAIA