Protein Info for QEN71_RS38800 in Paraburkholderia sabiae LMG 24235

Annotation: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 806 transmembrane" amino acids 12 to 39 (28 residues), see Phobius details amino acids 46 to 63 (18 residues), see Phobius details amino acids 69 to 86 (18 residues), see Phobius details amino acids 98 to 116 (19 residues), see Phobius details amino acids 128 to 148 (21 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 20 to 805 (786 residues), 1039.2 bits, see alignment E=0 PF13360: PQQ_2" amino acids 224 to 324 (101 residues), 22 bits, see alignment E=1.7e-08 amino acids 684 to 788 (105 residues), 25.8 bits, see alignment E=1.2e-09 PF01011: PQQ" amino acids 229 to 255 (27 residues), 24 bits, see alignment (E = 3.7e-09) amino acids 375 to 400 (26 residues), 20.6 bits, see alignment (E = 4.4e-08) amino acids 457 to 483 (27 residues), 30.2 bits, see alignment (E = 4.1e-11) amino acids 736 to 772 (37 residues), 29.1 bits, see alignment 8.7e-11

Best Hits

KEGG orthology group: K05358, quinate dehydrogenase (quinone) [EC: 1.1.5.8] (inferred from 61% identity to kva:Kvar_1995)

Predicted SEED Role

"Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25)" in subsystem Chorismate Synthesis or Quinate degradation (EC 1.1.99.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.8 or 1.1.99.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (806 amino acids)

>QEN71_RS38800 membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (Paraburkholderia sabiae LMG 24235)
MSKQPETKPISTAARVGLTLAGAIGLLLGLYFAIGGALLVARGGTWYYLLMGLAVCATGI
QLARRKASACAIFALVIVATMLWAVWESGFDFWPLQARIFMFTMIGMLLAPVYPMLRGFE
GKRPAKGAAWTAGLVLLACNALFVYGMFIPHGTFGTESSVASAQHDSGSGDWTAYGHSAG
GDRFAGATQIDRGNVKNLQVAWTYHTGDVPLSPTGGGSEDQETPLQIGDTLFVCSPHNTV
IALDAANGHEKWRHEFPTKTTVWVRCRGLAYFDATKPVQQPSVTGSTPVTPVALADEHAA
CRRRIFMNTIDAKLVALDADTGKLCTDFGDQGVIDLKAGLGSAASPLYELTSPPTVAGTT
VVTGGRVADNVTLDMPGGVIRGFDVMTGAMKWAFDPGNPQDKQAPAPGKTFVRSTPNVWA
PMSYDATSNTVYMPVGSAAIDLWGVKRTQLDEKYGASILALDATTGEEKWHFQTVHHDLW
DYDVPMQPTLVDFPVDGKNVPALIVGTKMGQLFVLDRLTGKPLTKVVEQPVKSATIPGEP
YAKTQPLSVGMPQIGAEVLKGADMWGMTPIDQMMCRIVFHGMRYDGLFTAPDTDTSLSFP
GSLGGMNWGGLSYDPNSQMIFANDMRLGLWVHLVKEEKRGGTSNGNEAVNAGMGAVPLGG
TPYSVTKDRFFSPLGIPCQKPPFGSLTAIDLKTRSIAWQVPLGTVRDTRLWGVQMHMPTP
IGMPTIGGSLSTGGGLVFFAATQDYYLRAFDSSTGKEVWKARLPVGSQGTPMSYVLNGKQ
YIVISAGGARQSPDRGDYVIAYALPQ