Protein Info for QEN71_RS34385 in Paraburkholderia sabiae LMG 24235

Annotation: 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 TIGR02299: 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase" amino acids 3 to 484 (482 residues), 788.8 bits, see alignment E=9.1e-242 PF00171: Aldedh" amino acids 15 to 471 (457 residues), 588.5 bits, see alignment E=3.9e-181

Best Hits

Swiss-Prot: 65% identical to HPCC_ECOLX: 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (hpcC) from Escherichia coli

KEGG orthology group: K00151, 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase [EC: 1.2.1.60] (inferred from 91% identity to bug:BC1001_5334)

MetaCyc: 67% identical to subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (Escherichia coli B)
5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase. [EC: 1.2.1.60]

Predicted SEED Role

"5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic amino acid degradation or Central meta-cleavage pathway of aromatic compound degradation (EC 1.2.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.60

Use Curated BLAST to search for 1.2.1.60

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (485 amino acids)

>QEN71_RS34385 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (Paraburkholderia sabiae LMG 24235)
MRIEHLINGKPKAAREYFETVNPATQEVLAEVASGTAEDIDAAVQAAKEAFPAWAAKPAS
ERAKLVRKLGELIAKNVPELSDTETRDTGQTISQTRKQLVPRAADNFTYFAEMCTRVDGH
TYPTDSHLNYTLFHPVGVCALISPWNVPFMTATWKVAPCLAFGNTAVLKMSELSPLTASM
LGTLALEAGIPAGVLNVVHGYGKDAGEPLVAHPDVHAISFTGSTATGNRIVQTAGLKKFS
MELGGKSPFVIFDDADFERALDAAVFMIFSNNGERCTAGSRILVQKSIYAKFAERFIERA
KRLTVGDPLSESTIIGPMISQAHLAKVRSYIELGPKEGATLACGGLDAPTLPEALKQGNF
VTPTVFVDVDNRMRIAQEEIFGPVACLIPFDDEAEAIRLANDISYGLSSYVWTENTGRAH
RVAAAIEAGMCFVNSQNVRDLRQPFGGTKASGVGREGGTWSYEVFLEPKNVCVSLGSHHI
PRWGV