Protein Info for QEN71_RS34280 in Paraburkholderia sabiae LMG 24235

Annotation: 4-hydroxyproline epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05544: Pro_racemase
  • amino acids 9 to 331 (323 residues), 374.2 bits, E=2.8e-116

Best Hits

Swiss-Prot: 74% identical to 4HPE2_BURM1: 4-hydroxyproline 2-epimerase 2 (prdF) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: None (inferred from 74% identity to bmu:Bmul_5265)

Predicted SEED Role

"4-hydroxyproline epimerase (EC 5.1.1.8)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 5.1.1.8)

MetaCyc Pathways

• trans-4-hydroxy-L-proline degradation II (4/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.1.8

Use Curated BLAST to search for 5.1.1.8

Protein Sequence (338 amino acids)

>QEN71_RS34280 4-hydroxyproline epimerase (Paraburkholderia sabiae LMG 24235)
MSRHRFVSIEGHTEGMPVRMLIDGPPALEGATMAARRKAFVESHDWIRRALMLEPRGHAH
MSGTLLYPPVSADADMSLLFIETSGCLPMCGHASIGSISFALEAGLVRPKSPGTVVVDVP
AGKLHARYEMDGERVASVRFTNVPSFLLHRDVEIAHPHFGRLTLDIAYGGNFYPIVEVQP
NFPGCEHFSPEQLLMWGRQLQQAVNETLDVVHPDDPAIRGIRHAMWTGAPISDDADARAV
VIAGDSLIDRSPCGTGTSARVAQRYARGMLAQREAFRHQSLIGSCFTGRVESTTRLGNGL
DAVFPSVEGRAWITGRAEYYVDDAQPYAHGFSLQEYAN