Protein Info for QEN71_RS34005 in Paraburkholderia sabiae LMG 24235

Annotation: aminotransferase class V-fold PLP-dependent enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 63% identical to SGAA_METEA: Serine--glyoxylate aminotransferase (sgaA) from Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)

KEGG orthology group: K00830, alanine-glyoxylate transaminase / serine-glyoxylate transaminase / serine-pyruvate transaminase [EC: 2.6.1.44 2.6.1.45 2.6.1.51] (inferred from 96% identity to bph:Bphy_5929)

MetaCyc: 59% identical to serine-glyoxylate aminotransferase subunit (Hyphomicrobium methylovorum GM2)
Serine--glyoxylate transaminase. [EC: 2.6.1.45]; 2.6.1.45 [EC: 2.6.1.45]

Predicted SEED Role

"Serine--glyoxylate aminotransferase (EC 2.6.1.45)" in subsystem Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle (EC 2.6.1.45)

MetaCyc Pathways

formaldehyde assimilation I (serine pathway) (12/13 steps found)
photorespiration II (8/10 steps found)
photorespiration I (7/9 steps found)
photorespiration III (7/9 steps found)
glycine biosynthesis III (1/1 steps found)
L-serine biosynthesis II (3/4 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.44, 2.6.1.45, 2.6.1.51

Use Curated BLAST to search for 2.6.1.44 or 2.6.1.45 or 2.6.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (421 amino acids)

>QEN71_RS34005 aminotransferase class V-fold PLP-dependent enzyme (Paraburkholderia sabiae LMG 24235)
MSNTSGSRIAGRNILAVPGPTNIPDAVLRAMVVSMEDHRSTKFPELAHGLLSDLKKIYRT
ADGQPFIFPSSGTGAWEAALTNTLSPGDRVLVPRFGQFSHLWADMAQRLGFDVEILDVDW
GEGVPVERIAEILKADGQHTIKGILACHNETATGVTSDIGALRRAMDAADHPALLFVDGV
SSIGCIDFRMDEWGVDLAVTGSQKGLMLPAGLGILCVSQKAMKAISHAKSRRCYFDLADM
VRTNATGYFPYTPALSLLYGLRAALDLLFEEGLENVFARHHYLASGVRAAVTEGWGLSLC
AKAPRWHSDTVSAIVVPEGVNGAQVIDTAYRRYNLALGAGLSKVAGKVFRIGHLGDLNEL
MLMSAIAGAEMAMLDAGIDVCPGSGVGAAGQYWRTHTPGAQAAPAGEQPAVEHVRRRAAA
A