Protein Info for QEN71_RS33800 in Paraburkholderia sabiae LMG 24235

Annotation: asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00520: L-asparaginase, type II
  • amino acids 30 to 369 (340 residues), 375.9 bits, E=8.6e-117
• PF00710: Asparaginase
  • amino acids 47 to 236 (190 residues), 207.2 bits, E=1.7e-65
• PF17763: Asparaginase_C
  • amino acids 257 to 368 (112 residues), 107 bits, E=5.8e-35

Best Hits

Swiss-Prot: 49% identical to ASPG_WOLSU: L-asparaginase (ansA) from Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)

KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 72% identity to bge:BC1002_6680)

Predicted SEED Role

"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)

MetaCyc Pathways

• L-asparagine degradation I (1/1 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
• L-asparagine degradation III (mammalian) (2/3 steps found)
• N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Cyanoamino acid metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.1

Use Curated BLAST to search for 3.5.1.1

Protein Sequence (372 amino acids)

>QEN71_RS33800 asparaginase (Paraburkholderia sabiae LMG 24235)
MPRLVTSSCLTVSALLIAACIALPLCSTAAQAADTATTSNSRQSLPRVIVLATGGTISGT
ADARSAIGYNSGERTGKQLIKDVPDIGKFATISAEQISNVGSQDMSDGIWFQLAKRIDEI
FARGEAEGVVITHGTDTMEETAFFLKNVLHSRQPVVLVGSMRPGGVVGADGPNNLLEAVE
VAASPQSKGRGVMVVMNDTIHDPRWITKTNTTSVQTFMSPNAGPIGYVDPASVRYVAPAT
EARPSGFTLPAAGQLPRVDIVYAHSNMDASQIDHAVADNAKGIIVAGVGDGDVSKAALSA
MERAAKMGIVVVRASRVGTGFVNRNVEIDDDKSGFVASLDLNPQKARLLTQLLIANGVTT
PAKVQDAFSTTY