Protein Info for QEN71_RS32850 in Paraburkholderia sabiae LMG 24235

Annotation: methanol/ethanol family PQQ-dependent dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 606 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 494 to 512 (19 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 25 to 559 (535 residues), 725.2 bits, see alignment E=2.4e-222 PF13360: PQQ_2" amino acids 69 to 225 (157 residues), 52.4 bits, see alignment E=9.1e-18 amino acids 475 to 533 (59 residues), 22.4 bits, see alignment 1.3e-08 PF01011: PQQ" amino acids 140 to 170 (31 residues), 29.1 bits, see alignment (E = 8.7e-11) amino acids 505 to 536 (32 residues), 22.8 bits, see alignment (E = 8.9e-09) PF13570: PQQ_3" amino acids 483 to 521 (39 residues), 22.4 bits, see alignment 2e-08

Best Hits

Swiss-Prot: 63% identical to XOXF_PARDE: Putative dehydrogenase XoxF (xoxF) from Paracoccus denitrificans

KEGG orthology group: None (inferred from 99% identity to bph:Bphy_6478)

Predicted SEED Role

"Methanol dehydrogenase large subunit protein (EC 1.1.99.8)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (606 amino acids)

>QEN71_RS32850 methanol/ethanol family PQQ-dependent dehydrogenase (Paraburkholderia sabiae LMG 24235)
MNVRTLVLGLAVLVATALNSFLAYADPQLDSLIKNPSNWAAQAGDYANHRYSPLKQVNES
NVGKLQVAWTMSTGVLRGHEGSPLVIGDTMYIHSPFPNKVIAINLKDQTFIWQYEPKQDA
SVISVMCCDTVNRGLAYGDGKIFLQQADTKLVALNAKTGEVVWTAQNGDPKAGETNTNAP
HVFGDKVLTGISGGEFGVRGRLIAYDIKTGKEAWKAYSTGPDNEMLLDPQQTMTWADGKM
QPVGADSSIKSWKGDQWKLGGGTTWGWYAWDPKLNLVYYGTGNPGTWNPTQRPGDNKWSM
SIFARDLNTGKAKWVYQMTPHDEWDYDGVNEMILADIQMNGKKTPAIVHFDRNGFGYTLN
RETGELLVAQKYDPAVNWADSVDLKSGLPIRNASYSTQKAGPDHNVKGICPAALGSKDQQ
PASFDPGSNLFLVPTNHVCMDYEPFDVDYVSGQPYVGATLSMYPGPNEKGAMGNFIAWDA
AKGKIVWSKPERFSVWSGALATAGGIVFYGTLEGYIKAVRIKDGKELWKFKTPSGIIGNV
FTYEYQGKQFIGVYSGIGGWAGIGMAAGLQKSTEGLGAVGGYRELAKYTALGGTLFIFAI
PGGTNG