Protein Info for QEN71_RS32720 in Paraburkholderia sabiae LMG 24235

Annotation: HisA/HisF-related TIM barrel protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00977: His_biosynth
  • amino acids 2 to 244 (243 residues), 62.2 bits, E=2.7e-21

Best Hits

KEGG orthology group: K01814, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [EC: 5.3.1.16] (inferred from 79% identity to bph:Bphy_6503)

Predicted SEED Role

"Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase related protein"

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• L-histidine biosynthesis (9/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Histidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.16

Use Curated BLAST to search for 5.3.1.16

Protein Sequence (259 amino acids)

>QEN71_RS32720 HisA/HisF-related TIM barrel protein (Paraburkholderia sabiae LMG 24235)
MQVIPVLDLLDGHAVRAIRGDRANYRPIQSSLCATSEPLDVAHALVAATSADALYVADLG
AIMSRGADPSTLAALAATLGASPDRSQPFEIWLDAGFADFPSMRAHLERTTAAQHEHAHN
VRIVPIFGSESLRSPDALREAEAAGYAPILSLDHRHGQLIAAPELVPALRSPASWPSRVI
VMTLDQVGADAGPDLDTFNHIHAQAAQRMTIGAGGIRHRDDLDAAARSGAHAWLVASAIH
DGKLGTTQAPQATRSDAAA