Protein Info for QEN71_RS32675 in Paraburkholderia sabiae LMG 24235

Annotation: pyrroloquinoline quinone biosynthesis protein PqqE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 TIGR02109: coenzyme PQQ biosynthesis enzyme PqqE" amino acids 19 to 366 (348 residues), 525.6 bits, see alignment E=5.5e-162 PF04055: Radical_SAM" amino acids 30 to 184 (155 residues), 87.3 bits, see alignment E=2e-28 PF13353: Fer4_12" amino acids 33 to 99 (67 residues), 24.2 bits, see alignment E=5.8e-09 PF13186: SPASM" amino acids 253 to 319 (67 residues), 35.3 bits, see alignment E=1.8e-12 TIGR04085: radical SAM additional 4Fe4S-binding SPASM domain" amino acids 260 to 346 (87 residues), 29.1 bits, see alignment E=1.1e-10

Best Hits

Swiss-Prot: 58% identical to PQQE_PSEU2: PqqA peptide cyclase (pqqE) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K06139, pyrroloquinoline quinone biosynthesis protein E (inferred from 94% identity to bph:Bphy_6512)

MetaCyc: 57% identical to glutamate Cgamma--tyrosine C3 ligase (Klebsiella pneumoniae)
RXN-11176 [EC: 1.21.98.4]

Predicted SEED Role

"Coenzyme PQQ synthesis protein E" in subsystem Coenzyme PQQ synthesis or Pyrroloquinoline Quinone biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.21.98.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (405 amino acids)

>QEN71_RS32675 pyrroloquinoline quinone biosynthesis protein PqqE (Paraburkholderia sabiae LMG 24235)
MTDLSIPSQEQAQQASRSSVGPPLWLLAELTYRCPLHCAFCYNPVNYTDHRDELSTAQWI
DVLQQARKLGAAQLGFSGGEPLMRDDLEELVAEAHRLGFYTNLITSGVGLTDARLDVLKA
NGLDHIQLSFQDSTQELNDFLSSTRTFDLKNRVARSIKAHGFPMVLNCVLHRYNLPHVDK
IIDMALAMGAEFLELANTQYYGWAHANRAQLMPTAEQLRDAEAVVERYRKEIGNRCKIFF
VVPDYFETRPKRCMNGWGSVFLGVAPDGAALPCHSARSLPGLTFPNVKDTSLEHIWFDSD
AFNRFRGFEWMKEPCRSCDEKTKDLGGCRCQAYLLTQDAANADPVCDKSAHHEQVVRVVR
EAANRPATLAPNEQPVTFRNDANSRRLTASAQEPTATPHNEGTRS