Protein Info for QEN71_RS31410 in Paraburkholderia sabiae LMG 24235

Annotation: NAD(P)-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01370: Epimerase
  • amino acids 12 to 173 (162 residues), 81.6 bits, E=1.8e-26
• PF02719: Polysacc_synt_2
  • amino acids 12 to 121 (110 residues), 25.9 bits, E=1.6e-09
• PF16363: GDP_Man_Dehyd
  • amino acids 13 to 168 (156 residues), 40.2 bits, E=9.3e-14
• PF01073: 3Beta_HSD
  • amino acids 14 to 151 (138 residues), 38.7 bits, E=1.9e-13
• PF13460: NAD_binding_10
  • amino acids 16 to 120 (105 residues), 31.2 bits, E=6.1e-11

Best Hits

Swiss-Prot: 52% identical to URODH_PSESM: Uronate dehydrogenase (udh) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 59% identity to rhi:NGR_b03070)

MetaCyc: 52% identical to uronic acid dehydrogenase subunit (Pseudomonas syringae)
Uronate dehydrogenase. [EC: 1.1.1.203]; 1.1.1.203 [EC: 1.1.1.203]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• D-glucuronate degradation III (2/2 steps found)
• D-glucuronate degradation II (4/5 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• D-galacturonate degradation III (1/2 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• D-galacturonate degradation II (3/5 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (19/31 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 1.1.1.203 or 5.1.3.2

Protein Sequence (277 amino acids)

>QEN71_RS31410 NAD(P)-dependent oxidoreductase (Paraburkholderia sabiae LMG 24235)
MTDISKPPFKNILITGAAGALGSVLRESLRPYAEQLTLSDRASLGEVRANERHFVCELAD
RNAVFEMMKGVDMVIHLGGAPRENTFQVILDSNIAGSYHIYEAARQEGVKRVIYASSVHA
IGFYECVETIDANVPQRPDSLYGVSKAFVENLSRYYFDKFGLESVCVRIGSCFPKPVDRR
MLATWLSYDDFSHLCIQALMTPSVGHMIVYGNSNNSANFWDNTQAGYLGYRPQDAADSYR
AEVYANTPIGDPKHPAVRFQGGQFAADGHFEDDPKNR