Protein Info for QEN71_RS29655 in Paraburkholderia sabiae LMG 24235

Annotation: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 PF10396: TrmE_N" amino acids 9 to 130 (122 residues), 123.9 bits, see alignment E=7.9e-40 TIGR00450: tRNA modification GTPase TrmE" amino acids 13 to 466 (454 residues), 328.4 bits, see alignment E=8.2e-102 PF12631: MnmE_helical" amino acids 133 to 463 (331 residues), 190 bits, see alignment E=1.2e-59 TIGR00231: small GTP-binding protein domain" amino acids 228 to 376 (149 residues), 86.2 bits, see alignment E=2.1e-28 PF02421: FeoB_N" amino acids 228 to 317 (90 residues), 38.9 bits, see alignment E=1.2e-13 PF01926: MMR_HSR1" amino acids 229 to 342 (114 residues), 90 bits, see alignment E=2.4e-29

Best Hits

Swiss-Prot: 91% identical to MNME_PARXL: tRNA modification GTPase MnmE (mnmE) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 96% identity to bph:Bphy_3096)

MetaCyc: 53% identical to 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (466 amino acids)

>QEN71_RS29655 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE (Paraburkholderia sabiae LMG 24235)
MLATDSDPIVAIATAPGRGGIGVVRISFGRAGEAAAQPMMQALTGQALAPRHASYVPFLD
SGGNALDRGIALYFPAPHSYTGEHVLELQGHGGPIVLQLVLQRCIDAGRAFGLRLAEPGE
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDIHALVEDVITLRMLV
EATLDFPEEEIDFLEAADARGKLARIRERLALVLSEARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHVIDTAGLRETEDEVEKIGIERTW
GEIERADVVLHLLDARSGMTGDDETIAARFPRGVPVVRVINKTDLTGVAPDVAKLNGSGD
ADLSEVRLSAKMGDGISLLRGELLRIAGWQAGAESVYLARERHLIALRAAQNHLGLAAEH
ADQNSQALDLFAEELRLAQDQLNSITGEFTSDDLLGVIFSRFCIGK